Antibodies for
Immunohistochemistry
A-1-Antitrypsin (Polyclonal)
Rabbit Polyclonal Antibody
Cat. No. Description
Volume
45123 IMPATH A-1-Antitrypsin RTU R (Poly)
50 Tests
44198 Alpha-1-Antitrypsin RTU R (Poly)
7 ml Ready To Use
44423 Alpha-1-Antitrypsin 0,1 R (Poly)
100 µl liquid Concentrated
44424 Alpha-1-Antitrypsin 1 R (Poly)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Tonsil
Stability
Up to 36 mo. at 2-8°C
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
The immunohistochemical staining of alpha-1-antitrypsin is considered to be very useful in the study of inherited AAT deficiency, benign and
malignant hepatic tumors, and yolk sac carcinomas. Positive staining for A-1-antitrypsin may also be used in detection of benign and malignant
lesions of an histiocytic nature. Sensitivity and specificity of the results have made this antibody a useful tool in the screening of patients with
cryptogenic cirrhosis or other forms of liver disease with portal fibrosis of uncertain etiology.
Liver: Malignant vs. Benign
A1AT Hep-Par1 Glypican-3 CD34
p53
AFP
pCEA
mCEA
TTF-1
Hepatocellular Carcinoma
-/+
+
+
+
+
-/+
+
-
+
Cytoplasmic
Hepatoblastoma
+
+
+
-
+
+
+
-
-
Benign Liver Nodules
+/-
+
-
-
-
-
-
-
+
Cytoplasmic
Soft Tissue Tumor
A1AT
CK
Cocktail
MS Actin SM Actin Desmin A1ACT S-100
CD99
TFE-3 Calretinin
PNET/ES
-
-/+
-
-
-
-
+
+
-
-
Desmoplastic Small Round
Cell
-
+
-
-
+
-
-
-
-
-
Alveolar Soft Part Sarcoma
-
-
+
+
-
-
-
-
+
-
PEComa
+
-
-
+
+/-
-
-
-
-
+
Fibrous Histiocytoma
+
-
-
-
-
+
-
-
-
-
Skin: Spindle Cell Tumors
A1AT
FLI-1
CD10 SM Actin HHV-8
CD99
CD34
S-100 Collagen IV
Spindle Cell Melanoma
-
+
-
-
-
-
-
+
-
Atypical Fibroxanthomas
+
-
+
+
-
+
-
-
-
Angiosarcoma
-
+
-
-
-
-
+
-
+/-
Glomus Tumor
-
-
-
+
-
-
+/-
-
+
Hemangioma
-
+
-
+
-
-
+
-
+
Kaposi's Sarcoma
-
+
-
+
+
-
+
-
+/-
Reference
1. Isaacson P, et al. Lancet. 1979; 2:964-965.
2. Palmer PE, et al. Am J Clin Pathol. 1974; 62:350-354.
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