Advanced Solutions
for Advanced Pathology
Alpha-Fetoprotein (Polyclonal)
Rabbit Polyclonal Antibody
Cat. No. Description
Volume
45163 IMPATH Alpha-Fetoprotein RTU R (Poly)
50 Tests
44195 Alpha Fetoprotein RTU R (Poly)
7 ml Ready To Use
44417 Alpha Fetoprotein 0,1 R (Poly)
100 µl liquid Concentrated
44418 Alpha Fetoprotein 1 R (Poly)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Fetal Liver
Stability
Up to 36 mo. at 2-8°C
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Positive staining with anti-AFP is seen in hepatocytes of fetal liver and hepatoma. Since only traces of AFP are found in adult serum, elevated
levels suggest either a benign or malignant lesion of the liver, a yolk sac carcinoma, or one of a few other tumors. Correspondingly, in conjunction
with elevated serum levels, AFP has been immunohistochemically demonstrated in yolk sac tumor of gonadal and extragonadal sites, in hepatic
malignancies, and a few other neoplasms. The antigen is not denatured by most fixatives.
Liver: Malignant vs. Benign
AFP Hep-Par1 Glypican-3 CD34
p53
A1AT
pCEA
mCEA
TTF-1
Hepatocellular Carcinoma
-/+
+
+
+
+
-/+
+
-
+
Cytoplasmic
Hepatoblastoma
+
+
+
-
+
+
+
-
-
Benign Liver Nodules
-
+
-
-
-
+/-
-
-
+
Cytoplasmic
Germ Cell Tumors
AFP
Oct-4 Vimentin EMA Inhibin
hPL
CD30 Glypican-3 CD117 PLAP
Seminoma
-
+
+
-
-
-
-
-
+
+
Embryonal Carcinoma
-
+
-
-
-
-
+
-
-
+
Choriocarcinoma
-
-
-/+
+
-
+
-
+
-
+
Yolk Sac Tumor
+
-
-
-
-
-
-
+
-
+
Granulosa Cell Tumor
-
-
+
-
+
-
-
-
-
-
Hypercalcaemic Small Cell
Carcinoma
-
-
-
+
-
-
-
-
-
-
Reference
1. Jacobsen GK, et al. Am J Surg Pathol. 1981; 5:257-66.
2. Peyrol S, et al. Digestion. 1978; 18:351-370.
3. Tsung SH. Arch Pathol Lab Med. 1977; 101:572-574.
4. Goodman ZD, et al. Cancer. 1985; 55:124-135.
5. Roth LM, et al. Cancer. 1976; 37:812-820.
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