Antibodies for
Immunohistochemistry
BCL2 (E17
†
)
Rabbit Monoclonal Antibody
Cat. No. Description
Volume
45165 IMPATH BCL2 RTU R (E17)
50 Tests
44205 BCL2 RTU R (E17)
7 ml Ready To Use
44435 BCL2 0,1 R (E17)
100 µl liquid Concentrated
44436 BCL2 1 R (E17)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Tonsil
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Anti-BCL2 has shown consistent negative reaction on reactive germinal centers and positive staining of neoplastic follicles in follicular lymphoma.
Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. This
antibody may also be used in distinguishing between those follicular lymphomas that express BCL2 protein and the small number in which the
neoplastic cells are BCL2 negative.
Cutaneous Neoplasm
BCL2
CD10
Androgen
Receptor
CK 20
CD34
Ber-EP4
CK 19
Basal Cell Carcinoma
+
+
+
-
-
+
+
Trichoepithelioma
+
-
-
+
+
+
+
Merkel Cell Carcinoma
+
-
-
+
-
+
+
Microcystic Adnexal
Carcinoma
+
+/-
-
-
-
-/+
Sebaceous Carcinoma
+/-
+/-
+
-
-
+
-
Sebaceous Adenoma
+
-
+
-
-
+
-
B-cell Lymphomas
BCL2
PAX-5
BCL6 Annexin A1 IgD
MUM1
CD10
CD23 Cyclin D1
Follicular
+
+
+
-
+
-
+
-
-
CLL/SLL
+
+
-
-
+
+
-
+
-
Mantle Cell
+
+
-
-
-/+
-/+
-
-
+
Marginal Zone
+
+
-
-
+
+
-
-
-
Lymphoplasmacytic
+
+
-
-
-
+
-
-
-
Diffuse Large Cell
+
+
+
-
-
+
-/+
-
-
Burkitt
-
+
+
-
-
-
+
-
-
Hairy Cell Leukemia
+
+
-
+
-
-
-
+(weak)/-
Reference
1. Pezzella F, et al. Am J Pathol. 1990; 137:225-232.
2. Moul JW, et al. Eur urol. 1999; 35(5-6):399-407.
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