Antibodies for
Immunohistochemistry
ALK Protein (ALK-1)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45162 IMPATH ALK Protein RTU M (ALK-1)
50 Tests
44194 ALK Protein RTU M (ALK-1)
7 ml Ready To Use
44415 ALK Protein 0,1 M (ALK-1)
100 µl liquid Concentrated
44416 ALK Protein 1 M (ALK-1)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic, Nuclear
Control
Anaplastic large cell lymphoma
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
3
/k
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
ALK-1 is a fusion protein which is detected in 50%-85% of ALK+, anaplastic large cell lymphomas (ALCL) and has been shown to indicate
improved prognosis in the ALK-1 (+) ALCL. Studies have demonstrated approximately 5%-10% of non-small cell lung carcinoma can express
ALK protein recognized by this antibody producing a cytoplasmic staining pattern.
Hodgkin vs. Non-Hodgkin Lymphomas
ALK-1 CD79a CD15
CD30 Fascin Granzyme
B
BCL6
PU.1
MUM1
EMA
Hodgkin Lymphoma,
Classic
-
-
+
+
+
-
-
-
+
-
Hodgkin Lymphoma,
Nodular Lymphocyte
Predominant
-
+
-
-
-
-
+
+
-/+
+
T-cell Rich LBCL
-
+
-
-
-
-
+
-
+
-
Anaplastic Large Cell
Lymphoma
+
-
-
+
-
+
+/-
-
-
+
Soft Tissue Tumor
ALK-1
CK
Cocktail
Calretinin MS Actin SM Actin S-100 TLE-1
CD56
CD99
TFE-3
Synovial Sarcoma
-
+
+/-
-
-
-
+
+
+
-
Epithelioid Sarcoma
-
+
-
-/+
-
-
-
-
-
-
Clear Cell Sarcoma
-
-
-
-
-
+
-
-
-
-
PNET/ES
-
-/+
-
-
-
+
-
-
+
-
Desmoplastic Small Round
Cell
-
+
-
-
-
-
-
-
-
-
Myxoid Chondrosarcoma
-
-
+
-
-
+/-
-
-
-
Alveolar Soft Part Sarcoma
-
-
-
+
+
-
-
-
-
+
PEComa
-
-
+
-
+
-
-
-
-
-
Fibrous Histiocytoma
-
-
-
-
-
-
-
-
-
-
Inflammatory
Myofibroblastic Tumor
+
-
-
+
+
-
-
-
-
-
Reference
1. Cataldo KA, et al. Am J Surg Pathol. 1999; 32(1):1386-1392.
2. Travis WD, et al. J Clin Oncol. 2013; 31:992-1001.
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