Antibodies for
Immunohistochemistry
Actin, Muscle Specific (HHF35)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45235 IMPATH Actin Muscle Specific RTU M (HHF35)
50 Tests
44345 Actin Muscle Specific RTU M (HHF35)
7 ml Ready To Use
44710 Actin Muscle Specific 0,1 M (HHF35)
100 µl liquid Concentrated
45040 Actin Muscle Specific 1 M (HHF35)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Skeletal Muscle
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
1
/K
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Actin is a major component of the cytoskeleton. This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive
with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive
components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes
of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of
non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative. This antibody is useful in the identification of rhabdoid
cellular elements.
Soft Tissue Sarcoma
MS Actin CK Cocktail
EMA SM Actin Desmin
CD56
CD34
TFE-3 Myogenin
Epithelioid Sarcoma
-/+
+
+
-
-
-
+
-
-
Alveolar Soft Part Sarcoma
+
-
-
+
-
-
-
+
-
Leiomyosarcoma
+
-/+
-/+
+
+
+
-/+
-
-
Rhabdomyosarcoma
-/+
-
-
-/+
+
+
-
-
+
Reference
1. Gown, et al. A. J. P. 1986; 125:191.
2. Schmidt R, et al. A. J. P. 1988; 131:199.
3. Azumi N, et al. Modern Pathology. 1988; 1:469-474.
4. Rangdaeng L, et al. Am J Clin Pathology. 1991; 96:32-45.
5. Tsukada T, et al. Am J Pathology. 1987; 127:389-402.
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