Advanced Solutions
for Advanced Pathology
Actin, Smooth Muscle (1A4)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45121 IMPATH Actin Smooth Muscle RTU M (1A4)
50 Tests
44196 Actin Smooth Muscle RTU M (1A4)
7 ml Ready To Use
44419 Actin Smooth Muscle 0,1 M (1A4)
100 µl liquid Concentrated
44420 Actin Smooth Muscle 1 M (1A4)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Appendix, Uterus, Vessel wall
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG/K
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Actin is a major component of the cytoskeleton and is present in most cell types. Anti-smooth muscle actin does not stain cardiac or skeletal
muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin in
making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results
whereas anti-muscle specific actin is positive. Leiomyosarcomas are positive with both anti-muscle specific actin and anti-smooth muscle actin.
Muscle Malignant Tumors
SM Actin MS Actin Myogenin PGP 9.5 Caldesmon Myoglobin Calponin CD57 Vimentin INI-1
Leiomyosarcoma
+
+
-
-
+
-
+
+/-
+
Rhabdomyosarcoma
-/+
-/+
+
+
-
+
-
-
+
+
Soft Tissue Neoplasms
SM Actin Calretinin TFE-3
CD56
CD34
CK
Cocktail
Desmin
MS Actin S-100 HMB-45
Leiomyosarcoma
+
-
-
+
-/+
-/+
+
+
-
-
PEComa
+
+
-
+
-
-
-
-
+
+
Clear Cell Sarcoma
-
-
-
-
-
-
-
-
+
+
Alveolar Soft Part Sarcoma
+
-
+
-
-
-
-
+
-
-
Spindle Cell Tumors
SM Actin β-Catenin PGP 9.5 ALK CD56
EMA
CK
Cocktail
Calponin MS Actin Desmin
Myofibroblastic Tumor
+
-
-
+
+
-
-
+
+
+
Endometrial Stromal Tumor
+
+/-
+
-
-
-
-
+
+
-
Smooth Muscle
+
-
-
-
-
-
-
+
+
+
Fibromatosis
+
+
+
-
-
-
-
-
-
-
Leiomyosarcoma
+
-
-
-
+
+/-
-/+
+
+
+
Reference
1. Cooke PH. J Cell Biol. 1976; 68:539-556.
2. Skalli O, et al. J Cell Biol. 1986; 103:2787-2796.
3. Gown AM, et al. J Cell Biol. 1985; 100:807-813.
4. Kuroda M. Biochem Biophys Acta. 1985; 843:20-213.
5. Lazarides E. J Histochem Cytochem. 1975; 223:507-528.
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