Advanced Solutions
for Advanced Pathology
ACTH (Polyclonal)
Rabbit Polyclonal Antibody
Cat. No. Description
Volume
45161 IMPATH ACTH RTU R (Poly)
50 Tests
44193 ACTH RTU R (Poly)
7 ml Ready To Use
44413 ACTH 0,1 R (Poly)
100 µl liquid Concentrated
44414 ACTH 1 R (Poly)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Pituitary
Stability
Up to 36 mo. at 2-8°C
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
ACTH is synthesized from pre-pro-opiomelanocortin (pre-POMC). The removal of the single peptide during translation produces the 267 amino
acid polypeptide POMC, which undergoes a series of post-translational modifications such as phosphorylation and glycosylation before it is
proteolytically cleaved by endopeptidases to yield various polypeptide fragments with varying physiological activity. These fragments include
ACTH, β-lipotropin, γ-lipotropin, Melanocyte Stimulating Hormone (MSH), and β-endorphin. POMC, ACTH, and β-lipotropin are secreted from
corticotropes in the anterior lobe (or adenohypophysis) of the pituitary gland in response to the hormone corticotropin-releasing hormone (CRH)
released by the hypothalamus. ACTH is also produced by cells of immune system (T-cells, B-cells, and macrophages) in response to stimuli
associated with stress.
Anti-ACTH is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with ACTH-producing cells
(corticotrophs). It also may react with other tumors (e.g. some small cell carcinomas of the lung) causing paraneoplastic syndromes by secreting
ACTH.
Pituitary Panel
ACTH
FSH
GH
LH
Prolactin
TSH
Pituitary
+
+
+
+
+
+
Reference
1. Pizarro CB, et al. Braz J Med Biol Res. 2004 Feb; 37(2):235-43.
2. Viacava P, et al. J Endocrinol Invest. 2003 Jan; 26(1):23-8.
3. Kageyama K, et al. Am J Med Sci. 2002 Dec; 324(6):326-30.
4. Fan X, et al. J Histochem Cytochem. 2002 Nov; 50(11):1509-16.
5. Japon MA, et al. J Clin Endocrinol Metab. 2002 Apr; 87(4):1879-84.
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