Advanced Solutions
for Advanced Pathology
BCL6
‡
(GI191E/A8)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45166 IMPATH BCL6 RTU M (GI191E/A8)
50 Tests
44206 BCL6 RTU M (GI191E/A8)
7 ml Ready To Use
44437 BCL6 0,1 M (GI191E/A8)
100 µl liquid Concentrated
44438 BCL6 1 M (GI191E/A8)
1 ml liquid Concentrated
‡
BCL6 is protected by U.S. patents 6,174,997 and 6,783,945 (Cancer Genetics, Inc.)
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Nuclear
Control
Tonsil
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
1
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) for AP 2-step
Polymer (Universal) 12 min
*Please refer to product insert for complete protocol.
Product Description
BCL6 is a transcriptional regulator gene which codes for a 706-amino-acid nuclear zinc finger protein. Antibodies to this protein stain the
germinal center cells (benign or malignant) in lymphoid follicles, and interfollicular malignant cells in follicular lymphoma, diffuse large B-cell
lymphomas, and Burkitt lymphoma, as well as the majority of the Reed-Sternberg cells in nodular lymphocyte predominant Hodgkin lymphoma.
Anti-BCL6 rarely stains mantle cell lymphoma and MALT lymphoma. BCL6 expression is seen in approximately 68% of ALK+ anaplastic large
cell lymphomas (ALCL) but 28% of ALK- ALCL, NK/T-cell lymphoma (27%), peripheral T-cell lymphoma, NOS (8.6%), and T-lymphoblastic
lymphoma (9.1%). BCL6 expression can also be observed in angioimmunoblastic T-cell lymphoma (66%-96%).
B-cell Lymphomas
BCL6
TRAcP Annexin A1 CD79a
BCL2
CD10
CD23 Cyclin D1 MUM1
Follicular
+
-
-
+
+
+
-
-
-
CLL/SLL
-
-
-
+
+
-
+
-
+
Mantle Cell
-
-
-
+
+
-
-
+
-/+
Marginal Zone
-
+/-
-
+
+
-
-
-
+
Lymphoplasmacytic
-
-
-
+
+
-
-
-
+
Diffuse Large Cell
+
-
-
+
+
-/+
-
-
+
Burkitt
+
-
-
+
-
+
-
-
-
Hairy Cell Leukemia
-
+
+
+
+
-
-
+(weak)/-
Hodgkin vs. Non-Hodgkin Lymphomas
BCL6 CD79a CD15
CD30 Fascin Granzyme
B
PU.1
MUM1 ALK-1
EMA
Hodgkin Lymphoma,
Classic
-
-
+
+
+
-
-
+
-
-
Hodgkin Lymphoma,
Nodular Lymphocyte
Predominant
+
+
-
-
-
-
+
-/+
-
+
T-cell Rich LBCL
+
+
-
-
-
-
-
+
-
-
Anaplastic Large Cell
Lymphoma
+/-
-
-
+
-
+
-
-
+
+
Angioimmunoblastic T-cell
Lymphoma
+
-
-
-
-
-
-
-
-
-
Reference
1. Dogan A, Badgi E, et al. Am J Surg Pathol. 2000; 24(6):846-852.
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