Antibodies for
Immunohistochemistry
WT1 (6F-H2)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45270 IMPATH WT-1 RTU M (6F-H2)
50 Tests
44410 Wilms’ Tumor RTU M (6F-H2)
7 ml Ready To Use
44839 Wilms’ Tumor 0,1 M (6F-H2)
100 µl liquid Concentrated
44840 Wilms’ Tumor 1 M (6F-H2)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Nuclear
Control
Kidney, Mesothelioma, Ovarian
serous carcinoma, Testis
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
1
/k
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
WT1 is a suppressor gene located on chromosome 11p13. Wilms’ Tumor protein (WT1) has been identified in proliferative mesothelial cells,
malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas
rarely stain positive with this antibody.
Pleura: Adenocarcinoma vs. Mesothelioma
WT1 Calretinin CK 5
D2-40 HBME-1 Caldesmon TAG-72 Ep-CAM E-cadherin TTF-1
Adenocarcinoma
-
-
-
-
-
-
+
+
+
+
Mesothelioma
+
+
+
+
+
+
-
-
-
-
Colon vs. Ovarian Carcinoma
WT1
CK 7
CK 20
CEA CDX-2
Villin CA19-9 Ep-CAM CA-125 CK 5&6
Ovarian Carcinoma, Serous
+
+
-
+
-
+
+
+
+
-
Ovarian Carcinoma,
Mucinous
-
+
-
-
+
+
+
+
-
Ovarian Endometrioid Ca
-/+
+
-
-
-
+/-
+
+
-
Colon Carcinoma
-
-
+
+
+
+
+
+
-
-
Small, Round Blue Cell Tumors
WT1 MS Actin SM Actin Myogenin CK
Cocktail
CD99
FLI-1
CD57 Vimentin PGP 9.5
Lymphoblastic Lymphoma
-
-
-
-
-
+
+
-
+
Leiomyosarcoma
-
+
+
-
-/+
-
-
+/-
+
-
Rhabdomyosarcoma
-
-/+
-/+
+
-
-
-
-
+
+
Neuroblastoma
-
-
-
-
-
-
-
+
+
+
Embryonal Carcinoma
-
-
-
-
+
-
-
+
-
+
PNET/ES
-
-
-
-
-/+
+
+
+
+
+
DSRCT
+
-
-
-
+
-
+
+/-
+
-
Reference
1. Tsuta K, et al. Appl Immunohistochem Mol Morphol. 2009; 17:126–130.
2. Marchevsky AM. Arch Pathol Lab Med. 2008; 132:397-401.
3. Ordonez NG. Am J Surg Pathol. 1998; 22:1203-1214.
4. Ordonez NG. Arch Pathol Lab Med. 2005; 129:1407–1414.
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