Antibodies for
Immunohistochemistry
Villin (CWWB1)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45158 IMPATH Villin RTU M (CWWB1)
50 Tests
44408 Villin RTU M (CWWB1)
7 ml Ready To Use
44835 Villin 0,1 M (CWWB1)
100 µl liquid Concentrated
44836 Villin 1 M (CWWB1)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic, Membranous
Control
Colon
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
1
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Villin is a 95 kD glycoprotein of microvilli associated with rootlet formation in gastrointestinal mucosal epithelium. Anti-villin labels the brush border
area in the gastrointestinal mucosal epithelium. This antibody has been useful in differentiating gastrointestinal adenocarcinoma, neuroendocrine
carcinomas, and ovarian adenocarcinomas from adenocarcinomas of other organs. This antibody also labels Merkel cells of the skin.
Carcinomas
Villin Hep-Par1 CK 7
CK 20
pCEA CK 5
p63
CD10
TTF-1 β-Catenin
Hepatocellular Carcinoma
-
+
-
-
+
-
-
+
+
(cytoplasmic)
-
Bladder Carcinoma
+
-
+
+
+
-
-
+
-
-
Salivary Gland Carcinoma
-
-
+
-
+
+
+
-
Lung Adenocarcinoma
-
-
+
-
+
-
-
+
-
Colorectal Adenocarcinoma
+
-
-
+
+
-
-
+
-
+
Prostate Adenocarcinoma
-
-
-
-
-
-
-
+
-
-
Cervical Carcinoma
-
-
+
-
+
-
-
-
-
Sweat Gland Carcinoma
-
-
+
-
+
+
+
-
Pancreatic Carcinoma
-
-
+
-
+
-
-
+/-
-
-
Gastric Carcinoma
+
-
+
-
+
-
-
-
-
Colon vs. Ovarian Carcinoma
Villin
CK 7
CK 20
CEA CDX-2 CA19-9 Ep-CAM WT1 CA-125 CK 5&6
Ovarian Carcinoma, Serous
+
+
-
+
-
+
+
+
+
-
Ovarian Carcinoma,
Mucinous
+
+
-
-
+
+
+
-
-
Colon Carcinoma
+
-
+
+
+
+
+
-
-
-
Reference
1. Werling RW, et al. Am J Surg. Path. 2003; 27(3):303-310.
2. Jainyou T, et al. Hum Pathol. 29:390-396.
3. Goldstein NS, et al. Am J Clin Pathol. 116:319-325.
4. Lau SK, et al. Hum Pathol. 33:1175-1181.
5. Tamboli P, et al. Arch Pathol Lab Med. 2002; 126:1057-1063.
6. Zhang PJ, et al. Arch Pathol Lab Med. 1999; 123:812-816.
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