Advanced Solutions
for Advanced Pathology
Tyrosinase (T311)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45157 IMPATH Tyrosinase RTU M (T311)
50 Tests
44405 Tyrosinase RTU M (T311)
7 ml Ready To Use
44829 Tyrosinase 0,1 M (T311)
100 µl liquid Concentrated
44830 Tyrosinase 1 M (T311)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Melanoma, Skin
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
2a
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Tyrosinase is an enzyme, amongst a family of enzymes, which is involved in the biosynthesis of melanin. Anti-tyrosinase has been found to be
quite specific for melanotic lesions such as malignant melanoma and melanotic neurofibroma. Essentially no carcinomas express this marker.
Melanotic Lesions
Tyrosinase S-100 SOX-10 HMB-45 MART-1 MiTF
CD63 Factor
XIIIa
WT1
NGFR
Adult Melanocytes
+
+
+
-
+
+
+
-
Junctional Nevus
+
+
+
+
+
+
-
-
+/-
Interdermal Nevus
+
+
+
-
+
+
-
-
+/-
Primary Melanoma
+
+
+
+
+
+
+
-
-
Metastatic Melanoma
+
+
+
+
+
+
+
-
+
-
Spindle Cell Melanoma
+
+
+
+
+
+
+
-
+
+
Angiomyolipoma
-
+
+
+
+
+
+
-
Adrenal Cortical
-
+
-
+
-
-
-
Intranodal Nevus Cells
+
+
+
-
+
+
-
-
Dermatofibroma
-
-
-
-
-
-
-
+
PEComa
Tyrosinase HMB-45 MART-1 CD63
S-100 SM Actin Calponin Caldesmon Desmin CD68
Angiomyolipoma
-
+
+
+
-
+
+
+
-
+
Lymphangiomyomatosis
-
+
+
+
-
+
+
+
-
-
Extrapulmonary Clear Cell
Tumor
-
+
+
+
+
+
-
-
-
-
Primary Cutaneous
PEComa
-
+
+
+
-
-
-
-
-
+/-
Pulmonary Clear Cell Sugar
Tumor
-
+
+
+
+/-
-
-
-
-
+/-
Reference
1. Kaufmann O, et al. Mod Pathol. 1998 Aug; 11(8):740-6.
2. Busam KJ, et al. Am J Dermatopathol. 2000 Jun; 22(3):237-41.
3. Jungbluth AA, et al. Pathol Res Pract. 2000; 196(4):235-42.
4. Meije CB, et al. J Pathol. 2000 Apr; 190(5):572-8.
5. Busam KJ, et al. Am J Dermatopathol. 2000 Jun; 22(3):237-41.
235
