Antibodies for
Immunohistochemistry
TTF-1 (8G7G3/1)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45265 IMPATH TTF-1 RTU M (8G7G3/1)
50 Tests
44401 TTF-1 RTU M (8G7G3/1)
7 ml Ready To Use
44821 TTF-1 0,1 M (8G7G3/1)
100 µl liquid Concentrated
44822 TTF-1 1 M (8G7G3/1)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Nuclear
Control
Lung Adenocarcinoma
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
1
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Anti-TTF-1 (Thyroid Transcription Factor 1) is useful in differentiating primary adenocarcinoma of the lung from metastatic carcinomas originating
in the breast, mediastinal germ cell tumors, and malignant mesothelioma. It can also be used to differentiate small cell lung carcinoma from
lymphoid infiltrates. Loss of TTF-1 expression in non-small cell lung carcinoma has been associated with aggressive behavior of such neoplasms.
TTF-1 labelling is also seen in thyroid malignancies.
Liver: Malignant vs. Benign
TTF-1 Hep-Par1 Glypican-3 CD34
p53
AFP
A1AT
pCEA
mCEA
Hepatocellular Carcinoma
+
(cytoplasmic)
+
+
+
+
-/+
-/+
+
-
Hepatoblastoma
-
+
+
-
+
+
+
+
-
Benign Liver Nodules
+
(cytoplasmic)
+
-
-
-
-
+/-
-
-
Thyroid: Malignant vs. Benign
TTF-1
Thyroglobulin
Calcitonin
CK 19
Galectin-3
HBME-1
Papillary Carcinoma
+
+
-
+
+
+
Follicular Carcinoma
+
+
-
-/+
+
+/-
Medullary Carcinoma
+
-
+
+/-
-
+
Benign Thyroid
+
+
-
-
-
-
Lung Small Cell Carcinoma vs. Merkel Cell Carcinoma
TTF-1
CEA
CK 20
Chromo-
granin A E-cadherin
Neuro-
filament
CD117
Synapto-
physin
Merkel Cell Carcinoma
-
-
+
+(nuclear)
+
+
+
+
Lung Small Cell Carcinoma
+
-
-
-
-
-
+/-
+
Breast vs. Lung vs. Prostate Carcinoma
TTF-1
GCDFP-15
Mammaglobin
PSA
Napsin A
Breast Carcinoma
-
+
+
-
-
Lung Carcinoma
+
-
-
-
+
Prostate Carcinoma
-
-
-
+
-
Reference
1. Ordonez NG. Appl Immunohistochem Mol Morphol. 2012; 20:429-44.
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