Advanced Solutions
for Advanced Pathology
TRAcP (9C5)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45267 IMPATH TRAcP RTU M (9C5)
50 Tests
44403 TRAcP RTU M (9C5)
7 ml Ready To Use
44825 TRAcP 0,1 M (9C5)
100 µl liquid Concentrated
44826 TRAcP 1 M (9C5)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Hairy Cell Leukemia
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
2b
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP 2-step
Polymer (Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Tartrate resistant acid phosphatase (TRAcP) is a basic, iron-binding protein with high activity towards phosphoproteins, ATP and 4 nitrophenyl
phosphate. Expression of TRAcP is reported to be increased in the spleen and monocytes of individuals with Gaucher’s disease, splenocytes and
circulating white cells of individuals with hairy cell leukemia, spleens of individuals with Hodgkin disease, and the sera of individuals undergoing
active bone turnover. Elevated levels are also reported to be associated with various B-cell and T-cell leukemias and lymphomas, placental
decidual cells, syncytiotrophoblasts, and some macrophages distributed throughout maternal and embryonic tissues.
The histochemical identification of hairy cell leukemia via tartrate-resistant acid phosphatase assay has been a standard for over two decades.
Anti-TRAcP labels the cells of hairy cell leukemia (HCL) with a high degree of sensitivity and specificity. Worthy also of mention in this regard
are anti-annexin A1 and anti-CD11c. Other cells stained with anti-TRAcP are tissue macrophages and osteoclasts, which also express abundant
TRAcP activity.
B-cell Lymphomas
TRAcP CD79a BCL2
BCL6
CD10
CD23 Cyclin D1 CD5
IgD MUM1
Follicular
-
+
+
+
+
-
-
-
+
-
CLL/SLL
-
+
+
-
-
+
-
+
+
+
Mantle Cell
-
+
+
-
-
-
+
+
+
-/+
Marginal Zone
+/-
+
+
-
-
-
-
-
-/+
+
Lymphoplasmacytic
-
+
+
-
-
-
-
-
-
+
Diffuse Large Cell
-
+
+
+
-/+
-
-
-/+
-
+
Burkitt
-
+
-
+
+
-
-
-
-
-
Hairy Cell Leukemia
+
+
+
-
-
-
+(weak)/-
-
-
Reference
1. Janckila AJ, et al. Blood. 1995 May 15; 85(10):2839-44.
2. Yaziji H, et al. Am J Clin Pathol. 1995 Oct; 104(4):397-402.
3. Janckila AJ, et al. J Histochem Cytochem. 1996 Mar; 44(3):235-44.
4. Janckila AJ, et al. Hybridoma. 1997 Apr; 16(2):175-82.
5. Hoyer JD, et al. Am J Clin Pathol. 1997 Sep; 108(3):308-15.
6. Janckila AJ, et al. Biotech Histochem. 1998 Nov; 73(6):316-24.
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