Advanced Solutions
for Advanced Pathology
Vimentin (V9)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45159 IMPATH Vimentin RTU M (V9)
50 Tests
44409 Vimentin RTU M (V9)
7 ml Ready To Use
44837 Vimentin 0,1 M (V9)
100 µl liquid Concentrated
44838 Vimentin 1 M (V9)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Lymph Node, Tonsil
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG/k
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP 2-step Polymer (Universal) or AP
2-step Polymer (Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Anti-vimentin is of limited value as a diagnostic tool; however, when used in combination with other antibodies (in panels) it is useful for the
subclassification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas
from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. This antibody
recognizes a 57 kD intermediate filament. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and
fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not
contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.
Small, Round Blue Cell Tumors
Vimentin MS Actin PGP 9.5 Myogenin CK
Cocktail
CD99
FLI-1
CD57
WT1
INI-1
Leiomyosarcoma
+
+
-
-
-/+
-
-
+/-
-
Rhabdomyosarcoma
+
-/+
+
+
-
-
-
-
-
+
Embryonal Carcinoma
-
-
+
-
+
-
-
+
-
+
PNET/ES
+
-
+
-
-/+
+
+
+
-
+
DSRCT
+
-
-
-
+
-
+
+/-
+
+
Kidney: Renal Epithelial Tumors
Vimentin
RCC
CD10
PAX-2 Ksp-cadherin Parvalbumin CD117
Ep-CAM
Clear Cell RCC
+
+
+
+
-
-
-
-
CNS Tumors
Vimentin
GFAP
NGFR
INI-1
S-100
CK Cocktail
PR
EMA
Astrocytoma
+
+
+
+
+
-
-
-
Glioblastoma
+
+
-
+
+
-
-
-
Oligodendriglioma
+
-
-
+
+
-
-
-
Ependymoma
-/+
+
+
+
+
-
-
-
Meningioma
+
-
-
+
-
-
+
+
Schwannoma
+
+
+
+
+
-
-
-
Reference
1. Ishii Y, et al. Clin Exp Immunol. 1984; 58:183-192.
2. Davey FR, et al. Am J Pathol. 1987; 129:54-63.
3. Lane EB, et al. Nature. 1983; 303:701-704.
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