Antibodies for
Immunohistochemistry
Tryptase (G3)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45268 IMPATH Tryptase RTU M (G3)
50 Tests
44404 Tryptase RTU M (G3)
7 ml Ready To Use
44827 Tryptase 0,1 M (G3)
100 µl liquid Concentrated
44828 Tryptase 1 M (G3)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Uterus
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
1
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are stored in mast cell secretory granules and
basophils. These enzymes are released into the extracellular environment, and are resistant to all known endogenous proteinase inhibitors.
Several tryptase genes are clustered on chromosome 16p13.3. There are two separate genes: alpha and beta 1. Beta tryptases appear to be the
main isoenzymes expressed in mast cells whereas in basophils, alpha tryptases predominate. Tryptases have been implicated as mediators in
the pathogenesis of asthma and other allergic and inflammatory disorders.
Anti-tryptase is a good marker for mast cells, basophils, and their derivatives. Mastocytosis is a term collectively used for a group of disorders in
which there is abnormal accumulation of mast cells in one or multiple organs. Anti-tryptase, combined with anti-CD2, anti-CD25, and anti-CD117,
can be useful in the differential diagnosis of reactive mast cell hyperplasia, myelogenous neoplasms, mast cell leukemia, and mastocytosis.
Mastocytosis
Tryptase
CD117
CD25
CD163
CD2
Systemic Mastocytosis
+
+
+
-
+
Mast Cell Leukemia
+
+
+
-
+
Reactive Mast Cells
+
+
-
+
-
Reference
1. Fiorucci L, Ascoli F. Cell Mol Life Sci. 2004 Jun; 61(11):1278-95.
2. Li CY. Leuk Res. 2001 Jul; 25(7):537-41.
3. Jordan JH, et al. Hum Pathol. 2001 May; 32(5):545-52.
4. Gordon LK, et al. Clin Immunol. 2000 Jan; 94(1):42-50.
5. Aoki M, et al. Int Arch Allergy Immunol. 2003 Mar; 130(3):216-23.
6. Roberts IS, Brenchley PE. J Clin Pathol. 2000 Nov; 53(11):858-62.
7. Ghott A, et al. Am J Surg Pathol. 2003 Jul; 27(7):1013-9.
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