Advanced Solutions
for Advanced Pathology
ZAP-70 (2F3.2)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45271 IMPATH ZAP-70 RTU M (2F3.2)
50 Tests
44411 Zap-70 RTU M (2F3.2)
7 ml Ready To Use
44841 Zap-70 0,1 M (2F3.2)
100 µl liquid Concentrated
44842 Zap-70 1 M (2F3.2)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Chronic lymphocytic leukemia/Small
lymphocytic lymphoma, Tonsil
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
2a
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
ZAP-70 is a 70 kD protein tyrosine kinase found in T-cells and natural killer cells. Control of this protein translation is via the IgVH gene. ZAP-70
protein is expressed in leukemic cells of approximately 25% of chronic lymphocytic leukemia (CLL) cases as well. Anti-ZAP-70 expression is
an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP-70 negative) and Ig-unmutated (anti-ZAP-70 positive) CLL
subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP-70 positive Ig-unmutated CLL cases have been shown
to have a poorer prognosis.
B-cell Lymphomas
ZAP-70 CD79a BCL2
BCL6
CD10
CD23 Cyclin D1 CD5
MUM1 Annexin
A1
Follicular
-
+
+
+
+
-
-
-
-
-
CLL/SLL
+/-
+
+
-
-
+
-
+
+
-
Mantle Cell
-
+
+
-
-
-
+
+
-/+
-
Marginal Zone
-
+
+
-
-
-
-
-
+
-
Lymphoplasmacytic
-
+
+
-
-
-
-
-
+
-
Diffuse Large Cell
-
+
+
+
-/+
-
-
-/+
+
-
Burkitt
-
+
-
+
+
-
-
-
-
-
Hairy Cell Leukemia
-
+
+
-
-
-
+(weak)/-
-
+
Reference
1. Wiestner A, et al. Blood. 2003 June 15; 101(12):4944-4951.
2. Crespo M, et al. N Engl J Med. 2003 May 1; 348(18):1764-1775.
3. Chen L, et al. Blood. 2002 Dec 15; 100(13):4609-14.
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