Advanced Solutions
for Advanced Pathology
Thyroglobulin (MRQ-41)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45263 IMPATH Thyroglobulin RTU M (MRQ-41)
50 Tests
44399 Thyroglobulin RTU M (MRQ-41)
7 ml Ready To Use
44817 Thyroglobulin 0,1 M (MRQ-41)
100 µl liquid Concentrated
44818 Thyroglobulin 1 M (MRQ-41)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Thyroid
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
1
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Thyroglobulin is the glycoprotein precursor of the iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3). Thyroglobulin is obtained
from the thyroid gland and exhibits the general properties of the globulins. Human thyroglobulin (hTG) is a high molecular weight glycoprotein
(605 kDa) found in the thyroid follicular cells. It plays a central role in the uptake, incorporation, and regulated biosynthesis of thyroid hormones.
Anti-thyroglobulin reacts with human thyroglobulin as demonstrated by a single band of immunoblotting in a lysate of human thyroid tissue. The
vast majority of follicular carcinomas of the thyroid will give positive immunoreactivity for anti-thyroglobulin even though sometimes only focally.
Poorly differentiated carcinomas of the thyroid are frequently anti-thyroglobulin negative. Adenocarcinomas of other-than-thyroid origin do not
react with this antibody. This antibody is useful in identification of thyroid carcinoma of the papillary and follicular types. Presence of thyroglobulin
in metastatic lesions establishes the thyroid origin of tumor. Anti-thyroglobulin, combined with anti-calcitonin, can identify medullary carcinomas
of the thyroid. Furthermore, anti-thyroglobulin, combined with anti-TTF1, can be a reliable marker to differentiate between primary thyroid and
lung neoplasms.
Thyroid: Malignant vs. Benign
Thyroglobulin
Calcitonin
CK 19
Galectin-3
TTF-1
HBME-1
Papillary Carcinoma
+
-
+
+
+
+
Follicular Carcinoma
+
-
-/+
+
+
+/-
Medullary Carcinoma
-
+
+/-
-
+
+
Benign Thyroid
+
-
-
-
+
-
Reference
1. Bejarano PA, et al. 2000 Sep; 8(3):189-94.
2. Bellet D, et al. J clin Endocrin Metab. 1983; 56:530-533.
3. Heffess CS, et al. Cancer. 2002 Nov; 195(9):1869-78.
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