Antibodies for
Immunohistochemistry
PAX-5 (EP156
†
)
Rabbit Monoclonal Antibody
Cat. No. Description
Volume
45642 PAX-5 RTU R (EP156)
7 ml Ready To Use
44366 PAX-5 0,1 R (EP156)
100 µl liquid Concentrated
45628 PAX-5 1 R (EP156)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Nuclear
Control
Tonsil
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
Product Description
PAX-5 encodes for B-cell-specific activator protein (BSAP), a marker for B-cells, including B-lymphoblastic neoplasms and maturation stage.
It is found in most cases of mature and precursor B-cell non-Hodgkin lymphomas/leukemias. In approximately 97% of cases of classic Hodgkin
lymphoma, Reed-Sternberg cells express PAX-5. PAX-5 is not detected in multiple myeloma and solitary plasmacytoma, making it useful for such
differentiation. Diffuse large B-cell lymphomas do express PAX-5, save for those with terminal B-cell differentiation. T-cell neoplasms do not stain
with anti-PAX-5. There is a strong association with CD20 expression.
B-cell Lymphomas
PAX-5
CD10
CD20 CD79a BCL2
BCL6
TCL1 Annexin
A1
CD23 Cyclin D1
Follicular
+
+
+
+
+
+
+
-
-
-
CLL/SLL
+
-
+
+
+
-
+
-
+
-
Mantle Cell
+
-
+
+
+
-
+
-
-
+
Marginal Zone BCL
+
-
+
+
+
-
-
-
-
-
Lymphoplasmacytic
+
-
+
+
+
-
+
-
-
-
Diffuse Large Cell
Lymphoma
+
+
+
+
+
+
-/+
-
-
Burkitt Lymphoma
+
+
+
+
-
+
+
-
-
Hairy Cell Leukemia
+
-
+
+
+
-
+
+
-
+(weak)/-
Lymphoblastic Lymphomas, B-cell vs. T-cell
PAX-5
TdT
CD10
CD20
CD19
CD3
CD5
CD7
CD117 CD1a
B-cell
+
+
+
+/-
+
-
-
-
-
+
T-cell
-
+
+/-
-
-
+
+/-
+/-
-/+
+/-
Reference
1. Torlakovic E, et al. Am J Surg Pathol. 2002 Oct; 26(10):1343-50.
2. Willenbrock K, et al. Lab Invest. 2002 Sep; 82(9):1103-9.
3. Falini B, et al. Blood. 2002 Jan 15; 99(2):409-26.
4. Blood. 2003 Feb 15; 101(4):1505-12.
196
