Antibodies for
Immunohistochemistry
Podoplanin (D2-40)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45327 IMPATH Podoplanin RTU M (D2-40)
50 Tests
44375 Podoplanin RTU M (D2-40)
7 ml Ready To Use
44769 Podoplanin 0,1 M (D2-40)
100 µl liquid Concentrated
44770 Podoplanin 1 M (D2-40)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Tonsil
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
1
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP 2-step Polymer (Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Podoplanin is a transmembrane mucoprotein (38 kD) recognized by the D2-40 monoclonal antibody. Podoplanin is selectively expressed in
lymphatic endothelium as well as lymphangiomas, Kaposi sarcomas, and in a subset of angiosarcomas with probable lymphatic differentiation.
Podoplanin has also been shown to be expressed in epithelioid mesotheliomas, hemangioblastomas, and seminomas.
Pleura: Adenocarcinoma vs. Mesothelioma
D2-40 Calretinin CK 5&6 HBME-1 WT1 Caldesmon CEA Ep-CAM TAG-72 TTF-1
Adenocarcinoma
-
-
-
-
-
-
+
+
+
+
Mesothelioma
+
+
+
+
+
+
-
-
-
-
Skin: Spindle Cell Tumors
D2-40
FLI-1
NGFR MS Actin Factor VIII
HHV-8 CK 8 & 18 CD34
CD31 Collagen
IV
Spindle Squamous Cell
Carcinoma
+
-
-
-
-
-
+
-
-
-
Spindle Cell Melanoma
+
+
+
-
-
-
-
-
-
-
Peripheral Nerve Sheath
+
-
-
+
-
-
-
-
-
-
Angiosarcoma
+/-
+
-
-
+
-
-
+
+
+/-
Kaposi's Sarcoma
+
+
-
-
+
+
-
+
+/-
+/-
Gonads: Germ Cell Tumors vs. Somatic Adenocarcinoma
D2-40
Oct-4
CK Cocktail
EMA
CD117
PLAP
Vimentin
Seminoma
+
+
-
-
+
+
+
Hypercalcaemic Small Cell
Carcinoma
+
-
+
+
-
-
-
Renal Cell Carcinoma vs. Hemangioblastoma
D2-40
FLI-1
CD31
CK Cocktail
CD10
Metastatic RCC
-
-
-
+
+
Hemangioblastoma
+
+
+
-
-
Reference
1. Ordonez N. Adv Anat Pathol. 2006 Mar; 13(2):83-8.
2. Ordonez N. Hum Pathol. 2005 Apr; 36(4):372-80.
3. Niakosari F, et al. Arch Dermatol. 2005 Apr; 141(4):440-4.
4. Galambos C, Nodit L. Pediatr Dev Pathol. 2005 Mar-Apr; 8(2):191-9.
5. Fukunaga M. Histopathology. 2005 Apr; 46(4):396-402.
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