Antibodies for
Immunohistochemistry
PD-1 (NAT105)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45323 IMPATH PD1 RTU M (NAT105)
50 Tests
44368 PD-1 RTU M (NAT105)
7 ml Ready To Use
44755 PD-1 0,1 M (NAT105)
100 µl liquid Concentrated
44756 PD-1 1 M (NAT105)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Lymph node, Tonsil
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
1
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP 2-step
Polymer (Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Programmed death-1 (PD-1) is expressed on activated T-cells, B-cells, and myeloid cells. Anti-PD-1 is a marker of angioimmunoblastic lymphoma
and suggests a unique cell of origin for this neoplasm. Unlike CD10 and BCL6, PD-1 is expressed by few B-cells, so anti-PD-1 may be a more
specific and useful diagnostic marker in angioimmunoblastic lymphoma. In addition, PD-1 expression provides evidence that angioimmunoblastic
lymphoma is a neoplasm derived from germinal center-associated T-cells. PD-1 expression in angioimmunoblastic lymphoma lends further
support to this model of T-cell oncogenesis, in which specific subtypes of T-cells may undergo neoplastic transformation and result in specific
distinct histologic, immunophenotypic, and clinical subtypes of T-cell neoplasia.
T-cell Lymphomas
PD-1
CD45
CD2
CD3
CD4
CD5
CD7
CD8
CD25 CD45RO
Angioimmunoblastic
+
+
+
+
+
+
+
-
+
+
Lymphoblastic
-
+
+/-
+
+/-
+
+
+/-
+
+
Subcutaneous
Panniculitis-Like
-
+
+
+
-
+
+
+/-
-
+
NK
-
+
+
+
-
-
-/+
-
+
+
Cutaneous
-/+
+
+
+
+
-
+
-
-
-
Peripheral, NOS
-
+
+
+
+/-
+/-
+/-
-/+
+
+
Mycosis Fungoides
-
+
+
+
+
+
-
-
+
+
Lymph Node
PD-1
S-100
CD1a
Lysozyme
CD21/CD35
Follicular Dendritic Cell
Sarcoma
-
-
+/-
-
+
Dermatopathic
Lymphadenitis
-
+
+
+
-
Reference
1. Bolstad AI, et al. Arthritis Rheum. 2003 Jan; 48(1):174-85.
2. Dorfman DM, et al. Am J Surg Pathol. 2006 Jul; 30(7):802-10.
3. Hamanishi J, et al. Proc Natl Acad Sci U S A. 2007 Feb 27; 104(9):3360-5. Epub 2007 Feb 21.
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