Advanced Solutions
for Advanced Pathology
PNL2 (PNL2)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45326 IMPATH PNL2 RTU M (PNL2)
50 Tests
44374 PNL2 RTU M (PNL2)
7 ml Ready To Use
44767 PNL2 0,1 M (PNL2)
100 µl liquid Concentrated
44768 PNL2 1 M (PNL2)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Melanoma
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
1
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Anti-PNL2 is a novel monoclonal antibody, which has recently been introduced as an immunohistochemical reagent to stain melanocytes and
tumors derived therefrom. Anti-PNL2 may be most useful because of its high sensitivity for metastatic melanoma (87%), as opposed to 76%
for anti-HMB-45 and 82% for anti-MART-1. Anti-PNL2 yields a strong cytoplasmic staining of skin and oral mucosal melanocytes, and staining
of granulocytes when used at high concentration. Anti-PNL2 labels intraepidermal nevi while the dermal component of compound nevi are
largely non-reactive with anti-PNL2. Antibodies against PNL2, MART-1 (Melan A) and HMB-45 stain most clear cell sarcoma cells and a few
cells in angiomyolipomas and lymphangioleiomyomatosis. Non-melanocytic lesions found to be positive with this marker include PEComas and
melanotic Schwannoma. Anti-PNL2 is a useful antibody for the identification of melanomas and clear cell sarcomas. Differential diagnosis is
aided by the results from a panel of antibodies, including antibodies against HMB-45, MART-1, tyrosinase, and MiTF.
Melanotic Lesions
PNL2
S-100 HMB-45 MART-1 Tyrosinase MiTF
CD63 Factor
XIIIa
WT1 KBA.62
Adult Melanocytes
+
+
-
+
+
+
+
-
+
Junctional Nevus
+
+
+
+
+
+
-
-
+/-
+
Interdermal Nevus
+
+
-
+
+
+
-
-
+/-
+
Primary Melanoma
+
+
+
+
+
+
+
-
+
Metastatic Melanoma
+
+
+
+
+
+
+
-
+
+
Spindle Cell Melanoma
+
+
+
+
+
+
+
-
+
+
Angiomyolipoma
+
+
+
+
-
+
+
-
-
Adrenal Cortical Lesions
-
+
-
+
-
-
-
-
-
Intranodal Nevus Cells
+
+
-
+
+
+
-
-
+
Dermatofibroma
-
-
-
-
-
-
-
+
-
Spindle Cell Melanoma vs. Epithelioid Peripheral Nerve Sheath Tumor
PNL2
S-100
HMB-45
Tyrosinase
NGFR
Collagen IV
Spindle Cell Melanoma
+
+
+
+
+
-
PNST
-
+
+
+
+
+
Adrenal Adenoma
+
+
+
-/+
+
Reference
1. Philippe Rochaix, et al. Mod Pathol. 2003; 16(5):481-490.
2. Klaus J, et al. Am J Surg Pathol. 2005 March, 29(3):400-406.
3. Luc G Morris, et al. Head Neck. 2008; 30:771-775
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