Antibodies for
Immunohistochemistry
P504s (13H4)
Rabbit Monoclonal Antibody
Cat. No. Description
Volume
45288 IMPATH P504s RTU R (13H4) RUO
50 Tests
44360 P504s RTU R (13H4) RUO
7 ml Ready To Use
44740 P504s 0,1 R (13H4) RUO
100 µl liquid Concentrated
44741 P504s 1 R (13H4) RUO
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Prostate Carcinoma
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP 2-step Polymer (Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Alpha-Methylacyl-CoA Racemase (also known as AMACR or P504s) is an essential enzyme in the β-oxidation of branched-chain fatty acids.
AMACR over-expression has been demonstrated in several cancers including colorectal, prostate, bladder, renal cell carcinomas, and lymphoma.
Staining with the antibody to this enzyme has been useful in identifying prostate carcinoma and prostatic intraepithelial neoplasia, as well as
atypical adenomatous hyperplasia in formalin-fixed, paraffinized tissue in morphologically difficult cases.
Prostate: Malignant vs. Benign
P504s
PSA/PSAP Androgen
Receptor
CK, 34βE12
p63
CK 5
CK 14
Prostate Carcinoma
+
+
+
-
-
-
-
Benign Prostate
-/+
+
+
+
+
+
+
Prostate Lesions
P504s
PSA/PSAP CK, 34βE12
p63
CK 7
Thrombo-
modulin Uroplakin III
PAX-2
Prostate Carcinoma
+
+
-
-
-
-
-
-
Urothelial Carcinoma
-
-
+
+
+
+
+
-
Nephrogenic Adenoma
+
-
+/-
-
+
-
-
+
Colon vs. Prostate Adenocarcinoma
P504s
CDX-2
CK 20
CEA
CA19-9
PSA
Colon Adenocarcinoma
+
+
+
+
+
-
Prostate Adenocarcinoma
+
-
-
-
-
+
Reference
1. Browne TJ, et al. Hum Pathol. 2004 Dec; 35(12):1462-8.
2. Wu CL, et al. Hum Pathol. 2004 Aug; 35(8):1008-13.
3. Evans AJ. J Clin Pthol. 2003 Dec; 56(12):892-7.
4. Beach R, et al. Am J Surg Pathol. 2002 Dec; 26(12):1588-96.
5. Jiang Z, et al. Am J Surg Pathol. 2002 Sep; 26(9):1169-74.
6. Jiang Z, et al. Am J Surg Pathol. 2001 Nov; 25(11):1397-404.
7. Zhou M, et al. Am J Surg Pathol. 2002 Jul; 26(7):926-31.
8. Jun Luo, et al. Cancer Research. 2002 April 15; 62, 2220-2226.
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