Antibodies for
Immunohistochemistry
PLAP (NB10)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45252 IMPATH PLAP RTU M (NB10)
50 Tests
44371 PLAP RTU M (NB10)
7 ml Ready To Use
44761 PLAP 0,1 M (NB10)
100 µl liquid Concentrated
44762 PLAP 1 M (NB10)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Placenta
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG/k
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Anti-PLAP immunoreacts with germ cell tumors and can discriminate between these and other neoplasms. Somatic neoplasms e.g. breast,
gastrointestinal, prostatic, and urinary cancers may also immunoreact with antibodies to PLAP. Anti-PLAP positivity in conjunction with
anti-keratin negativity favors seminoma over carcinoma. Germ cell tumors are usually anti-keratin positive, but they regularly fail to stain with
anti-EMA, whereas most carcinomas stain with anti-EMA. Anti-PLAP has been useful in the diagnosis of gestational trophoblastic disease. This
antibody has shown cross-reaction with human intestinal alkaline phosphatase.
Germ Cell Tumors
PLAP Oct-4
AFP Vimentin EMA Inhibin
hPL
CD30 Glypican-3 CD117
Seminoma
+
+
-
+
-
-
-
-
-
+
Embryonal Carcinoma
+
+
-
-
-
-
-
+
-
-
Choriocarcinoma
+
-
-
-/+
+
-
+
-
+
-
Yolk Sac Tumor
+
-
+
-
-
-
-
-
+
-
Granulosa Cell Tumor
-
-
-
+
-
+
-
-
-
-
Hypercalcaemic Small Cell
Carcinoma
-
-
-
-
+
-
-
-
-
-
Uterus: Trophoblastic Proliferations
PLAP
p57
hCG
hPL
CK Cocktail
Vimentin
Partial Mole
+
+
Weak, diffuse
Weak, diffuse
Strong, diffuse
-
Complete Mole
Weak, focal
-
Strong, diffuse
Weak, focal
Strong, diffuse
-
Choriocarcinoma
Weak, focal
-
Strong, diffuse
Weak, focal
Strong, diffuse
-/+
Placental Site Tumor
Strong, diffuse
Strong, focal
Strong, diffuse Strong, diffuse Strong, diffuse
Reference
1. Jacobsen GK, et al. Acta Path Microb Immuno Scand Sect A. 1984; 92:323-329.
2. Paiva J, et al. Am J Pathol. 1984; 111:156-165.
3. Burke, AP, et al. Hum Path. 1988; 19:663-670.
4. Manivel JC, et al. Am J Surg Path. 1987; 11:21-29.
5. Wick MR, et al. Hum Path. 1987; 18:946-954.
6. Saad RS, et al. Appl Immunohistochem Mol Morphol. 2003 Jun; 11(2):107-12.
7. Goldsmith JD, et al. Am J Surg Pathol. 2002 Dec; 26(12):1627-33.
8. Losh A, Kainz C. Acta Obstet Gynecol Scand. 1996 Sep; 7598):753-6.
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