Advanced Solutions
for Advanced Pathology
p120 Catenin (MRQ-5)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45320 IMPATH p120 Catenin RTU M (MRQ-5)
50 Tests
44357 p120 Catenin RTU M (MRQ-5)
7 ml Ready To Use
44734 p120 Catenin 0,1 M (MRQ-5)
100 µl liquid Concentrated
44735 p120 Catenin 1 M (MRQ-5)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic, Membranous
Control
Lobular Breast Carcinoma
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
1
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP 2-step Polymer (Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
p120 catenin is encoded on chromosome 11q11. Alpha-catenin and beta-catenin bind to the intracellular domain of E-cadherin while p120
catenin binds E-cadherin at a juxta-membrane site. The complex stabilizes tight junctions. In the cell, p120 catenin localized to the E-cadherin/
catenins cell adhesion complex, directly associates with cytoplasmic C-terminus of E-cadherin and may similarly interact with other cadherins. A
deficiency of E-cadherin results in the intracytoplasmic accumulation of p120 catenin. Lobular carcinoma of the breast shows intracytoplasmic
accumulation of p120 catenin while ductal carcinoma shows reduced membrane p120 catenin without cytoplasmic accumulation. In gastric and
colonic carcinoma, strong cytoplasmic p120 catenin is associated with discohesive infiltrative morphology.
Breast Lesion
p120
GCDFP-15
Mammaglobin
β-Catenin
E-cadherin
CK, 34βE12
Lobular
+(cytoplasmic)
+
+
-
-
+
Ductal
+(membranous)
+
+
+(membranous)
+
-
Reference
1. Reynolds AB, et al. Oncogene. 1992; 7:2439-2445.
2. Thoreson MA, et al. Mol Cell Biol. 1994; 14:8333-8342.
3. Daniel JM, et al. Hybridoma. 2001 May; 20:159-165.
4. Mayerle J, et al. Gastroenterology. 2003; 124:949-960.
5. Sarrio D, et al. Oncogene. 2004 Apr 22; 23(19):3272-83.
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