Antibodies for
Immunohistochemistry
PGP 9.5 (Polyclonal)
Rabbit Polyclonal Antibody
Cat. No. Description
Volume
45256 IMPATH PGP9.5 RTU R (Poly)
50 Tests
44380 PGP9.5 RTU R (Poly)
7 ml Ready To Use
44779 PGP9.5 0,1 R (Poly)
100 µl liquid Concentrated
44780 PGP9.5 1 R (Poly)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Nerve Tissue
Stability
Up to 36 mo. at 2-8°C
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Protein gene product 9.5 (PGP 9.5), also known as ubiquitin carboxyl-terminal hydrolase-1 (UCHL-1), is a 27 kDa protein originally isolated from
whole brain extracts. Although PGP 9.5 expression in normal tissues was originally felt to be strictly confined to neurons and neuroendocrine
cells, it has been subsequently documented in distal renal tubular epithelium, spermatogonia, Leydig cells, oocytes, melanocytes, prostatic
secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells, and dermal fibroblasts. LK Campbell et al.
demonstrated immunostaining of a plethora of different mesenchymal neoplasms with this antibody.
Retroperitoneal Lesions
PGP 9.5
NSE
Synaptophysin Chromogranin
A
Neurofilament
S-100
GFAP
Neuroblastoma
+
+
+
+
+
-
+/-
Ganglioneuroblastoma
+
+
+
+
+
+
+
Ganglioneuroma
+
+
+
+
+
+
+
Small, Round Blue Cell Tumors
PGP 9.5 MS Actin Myoglobin Myogenin CK Cocktail
CD99
FLI-1
Vimentin
CD57
Rhabdomyosarcoma
+
-/+
+
+
-
-
-
+
-
Neuroblastoma
+
-
-
-
-
-
-
+
+
Embryonal Carcinoma
+
-
-
-
+
-
-
-
+
PNET/ES
+
-
-
-
-/+
+
+
+
+
Spindle Cell Tumors
PGP 9.5 β-Catenin MS Actin SM Actin CD56
EMA
CK
Cocktail
Calponin BCL2
S-100
Spindle Cell Carcinoma
+
+/-
-
-
-
+/-
+
-
-
-
Neurofibroma
+
-
-
-
+
-
-
-
+
+
Endometrial Stromal Tumor
+
+/-
+
+
-
-
-
+
-
-
Fibromatosis
+
+
-
+
-
-
-
-
-
-
Reference
1. Bassotti G, et al. J Clin Pathol. 2005 Sep; 58(9):973-7.
2. Campbell LK, et al. Mod Pathol. 2003 Oct; 16(10):963-9.
3. Kasprzak A, et al. Pol J Pathol. 2007; 58(1):23-33. Review.
4. Mahalingam M, et al. J Cutan Pathol. 2001 Jul; 28(6):282-6.
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