Antibodies for
Immunohistochemistry
p27
Kip1
(SX53G8)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
44359 p27 RTU M (SX53G8)
7 ml Ready To Use
44738 p27 0,1 M (SX53G8)
100 µl liquid Concentrated
44739 p27 1 M (SX53G8)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Nuclear
Control
Tonsil
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
1
/k
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
Product Description
Cyclin-dependent kinase inhibitor 1B (p27, Kip1), also known as CDKN1B, is a human gene, which encodes a protein belonging to the Cip/Kip
family of cyclin dependent kinase (Cdk) inhibitors. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4
complexes and thus controls the cell cycle progression at G1. It is often referred to as a cell cycle inhibitor protein because its major function is
to stop or slow down the cell division cycle.
Studies have shown that low p27 expression has been associated with unfavorable prognosis in renal cell carcinoma, colon carcinoma, breast
carcinomas, non-small-cell lung carcinoma, hepatocellular carcinoma, multiple myeloma, and lymph node metastases in papillary carcinoma of
the thyroid, as well as a more aggressive phenotype in carcinoma of the cervix.
Thyroid: Malignant vs. Benign
p27
Thyroglobulin Calcitonin
CK 19
Galectin-3
TTF-1
HBME-1
Papillary Carcinoma
-/+
+
-
+
+
+
+
Follicular Carcinoma
-
+
-
-
+
+
+/-
Medullary Carcinoma
+/-
-
+
+
-
+
+
Benign Thyroid
+
+
-
-
-
+
-
Reference
1. Lloyd Ricardo V, et al. Am J Pathol. 1999; 154:313-323.
2. Migita Toshiro, et al. Cancer. 2002; 94:973-9.
3. Haitel Andrea, et al. Urology. 2001; 58:477-481.
4. Tan Puay, et al. Cancer Research. 1997 April 1; 57:1259-1263.
5. Esposito Vincenzo, et al. Cancer Research. 1997 August 15; 57:3381-3385.
6. Khoo Mark L. C., et al. J Clin Endocrinol Metab. 2002; 87:1814-1818.
7. Huang, Lee-Wen et al. Gynecologic Oncology. 2002; 85:524-528.
8. Khoo Mark L., et al. Arch Otolaryngol Head Neck Surg. 2002; 128:253-257.
9. Ribal MJ, et al. Anticancer Res. 2003 Nov-Dec; 23(6D):5101-6.
10. Freedland SJ, et al. Urology. 2003 Jun; 61(6):1187-92.
11. Armengol C, et al. J Hepatol. 2003 May; 38(5):591-7.
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