Advanced Solutions
for Advanced Pathology
PU.1 (EPR3158Y
†
)
Rabbit Monoclonal Antibody
Cat. No. Description
Volume
44381 PU.1RTU R (EPR3158Y)
7 ml Ready To Use
44781 PU.1 0,1 R (EPR3158Y)
100 µl liquid Concentrated
44782 PU.1 1 R (EPR3158Y)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Nuclear
Control
Tonsil
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
Product Description
PU.1 is a transcription factor that has been shown to be important for normal B-cell development. PU.1 belongs to the Ets family of transcription
factors. It is expressed in the myeloid lineage and in immature as well as mature B-lymphocytes, with the exception of plasma cells. PU.1
is essential during early B-cell differentiation. The absence of PU.1 results in total block of B-cell development at the pre-pro stage. PU.1 is
expressed in germinal center B-cells and mantle B-cells. Various lymphomas are also positive for anti-PU.1 including the following: B-chronic
lymphocytic leukemia, mantle cell lymphoma, follicular lymphoma, marginal zone lymphoma, diffuse large cell lymphoma, diffuse large B-cell
lymphoma, and nodular lymphocyte predominant Hodgkin lymphoma. Torlakovic et al. have demonstrated a quantitative positive association
between a high level of expression of germinal center antigens (including PU.1) and a longer overall survival and progression-free survival in the
case of follicular lymphoma.
B-cell Lymphomas
PU.1
CD79a BCL6
Oct-2
CD5
MUM1 CD10
CD23 Cyclin D1 TRAcP
Follicular
+
+
+
+
-
-
+
-
-
-
CLL/SLL
+
+
-
+
+
+
-
+
-
-
Mantle Cell
+
+
-
+
+
-/+
-
-
+
-
Marginal Zone
+
+
-
+
-
+
-
-
-
+/-
Lymphoplasmacytic
+
-
-
+
-
-
-
-
Diffuse Large Cell
+
+
+
+
-/+
+
-/+
-
-
-
Burkitt
+
+
-
-
+
-
-
-
Hairy Cell Leukemia
+
-
-
-
-
+(weak)/-
+
Hodgkin vs. Non-Hodgkin Lymphomas
PU.1
CD79a CD15
CD30 Fascin Granzyme
B
BCL6 MUM1 ALK-1
EMA
Hodgkin Lymphoma,
Classic
-
-
+
+
+
-
-
+
-
-
Hodgkin Lymphoma,
Nodular Lymphocyte
Predominant
+
+
-
-
-
-
+
-/+
-
+
T-cell Rich LBCL
-
+
-
-
-
-
+
+
-
-
Anaplastic Large Cell
Lymphoma
-
-
-
+
-
+
+/-
-
+
+
Reference
1. Hoefnagel JJ, et al. Mod Pathol. 2006 Sep; 19(9):1270-6. Epub 2006 Jun 16.
2. Hromas R, et al. Blood. 1993 Nov 15; 82(10):2998-3004.
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