Advanced Solutions
for Advanced Pathology
BG8, Lewis
Y
(F3)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45294 IMPATH BG8 RTU M (F3)
50 Tests
44208 BG8 RTU M (F3)
7 ml Ready To Use
44441 BG8 0,1 M (F3)
100 µl liquid Concentrated
44442 BG8 1 M (F3)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Lung adenocarcinoma
Stability
Up to 36 mo. at 2-8°C
Isotype
IgM
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Blood group antigens have been examined as potential discriminators between pulmonary adenocarcinoma (PACA) and epithelioid mesotheloma
(EM). Lewisy is the only one of these that appears to have some merit. BG8 is raised from the SKLU-3 lung cancer line and its ability to distinguish
between PACA and EM was first reported by Jordon and colleagues in 1989. Three groups have since reported their results. These studies
included 231 cases of PACA and 197 cases of EM. Sensitivity and specificity for PACA were both 93%. Yaziji H et al. reported a senstitivy of
nonmesothelial antigens for adenocarcinoma as organ dependent, with BG8 performing at 98% in the breast cancer group, and 100% in the
lung cancer group. They concluded using logical regression analysis that a three-antibody immunohistochemical panel including anti-calretinin,
anti-BG8, and anti-MOC-31 (Ep-CAM) would provide 96% sensitivity and 100% specificity for distinguishing EM from adenocarcinoma from
various sources (lung, ovary, breast, stomach).
Thymus
BG8
CK 5
p63
BCL2
CD117
CD5
CD1a
CD57
Thymic Carcinoma
+
+
+
+
+
+
-
-
Thymoma
-
+
+
-
-
-
+
+
Pleura: Adenocarcinoma vs. Mesothelioma
BG8
Calretinin CK 5&6
D2-40 Caldesmon E-cadherin TTF-1
TAG-72 Ep-CAM
Adenocarcinoma
+
-
-
-
-
+
+
+
+
Mesothelioma
-
+
+
+
+
-
-
-
-
Skin: Spindle Cell Tumors
BG8
FLI-1 Factor VIII
HHV-8 CK 8 & 18 CD34 SM Actin NGFR CD10
S-100
Spindle Squamous Cell Ca
-
-
-
-
+
-
-
-
-
-
Spindle Cell Melanoma
-
+
-
-
-
-
-
+
-
+
Atypical Fibroxanthomas
-
-
-
-
-
-
+
-
+
-
DF-SP
-
-
-
-
-
+
-
+
+/-
-
Peripheral Nerve Sheath
-
-
-
-
-
-
-
-
-
+/-
Angiosarcoma
-
+
+
-
-
+
-
-
-
-
Hemangioma
+
+
+
-
-
+
+
-
-
-
Kaposi's Sarcoma
-
+
+
+
-
+
+
-
-
-
Reference
1. King JE, et al. Histopathology. 2006 Feb; 48(3):223-32. Review.
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