Antibodies for
Immunohistochemistry
Thrombomodulin (1009)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
44398 Thrombomodulin RTU M (1009)
7 ml Ready To Use
44815 Thrombomodulin 0,1 M (1009)
100 µl liquid Concentrated
44816 Thrombomodulin 1 M (1009)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic, Membranous
Control
Bladder, Mesothelioma
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
1
/k
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
Product Description
Thrombomodulin (TM) is a transmembrane glycoprotein composed of 575 amino acids (molecular weight 75 kD) with natural anticoagulant
properties. It is normally expressed by a restricted number of cells such as endothelial and mesothelial cells. In addition, synovial lining
and syncytiotrophoblasts of human placenta also express TM. Several immunohistochemical endothelial markers are currently available and
anti-thrombomodulin serves as another such marker, staining blood and lymphatic channels consistently. Anti-TM has demonstrated positivity
in 100% of benign vascular tumors (pyogenic granuloma and hemangioma) and 94% of malignant vascular tumors (Kaposi’s sarcoma,
angiosarcoma, and epithelioid hemangioendothelioma). Hence, anti-TM serves as a sensitive marker for lymphatic endothelial cells and their
tumors. There has also been recent interest in the use of anti-TM as an immunohistochemical marker for mesothelial cells and malignant
mesotheliomas. Anti-TM is immunoexpressed in a variety of other tumors including squamous cell carcinomas of the lung, synovial sarcoma,
transitional cell carcinoma, renal cell carcinomas, and thymomas.
Pleura: Adenocarcinoma vs. Mesothelioma
Thrombo-
modulin Calretinin CK 5&6 HBME-1 Caldesmon CEA TAG-72 Ep-CAM E-cadherin TTF-1
Adenocarcinoma
-
-
-
-
-
+
+
+
+
+
Mesothelioma
+
+
+
+
+
-
-
-
-
-
Squamous vs. Transitional Cell Carcinoma
Thrombo-
modulin
CK, 34βE12
p63
CK 5
CK 7
CK 20
Uroplakin III
Squamous Carcinoma
+
+
+
+
-
-
-
Transitional Cell Carcinoma
+
+
+
-/+
+
+
+
Prostate Lesions
Thrombo-
modulin PSA/PSAP P504s
CK, 34βE12
p63
CK 7
Uroplakin III
PAX-2
Prostate Carcinoma
-
+
+
-
-
-
-
-
Urothelial Carcinoma
+
-
-
+
+
+
+
-
Nephrogenic Adenoma
-
-
+
+/-
-
+
-
+
Reference
1. Acebo E, et al. Histology and histopathology. 2001; 16:1031-6.
2. Appleton MAC, et al. Histopathology. 1996; 29:153-7.
3. Attanoos RL, et al. Histopathology. 1996; 29:209-15.
4. Attanoos RL, et al. Histopathology. 2001; 39:584-8.
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