Advanced Solutions
for Advanced Pathology
Cyclin D1 (EP12
†
)
Rabbit Monoclonal Antibody
Cat. No. Description
Volume
44263 Cyclin D1 RTU R (EP12)
7 ml Ready To Use
44547 Cyclin D1 0,1 R (EP12)
100 µl liquid Concentrated
44548 Cyclin D1 1 R (EP12)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Nuclear
Control
Mantle Cell Lymphoma
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
Product Description
Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. Cyclins are
proteins that govern transitions through distinct phases of the cell cycle by regulating the activity of the cyclin-dependent kinases. In mid-to-late
G1 phase of the cell cycle, cyclin D1 shows a maximum expression following growth factor stimulation. Anti-cyclin D1 has been successfully
employed and is a promising tool for further studies in both cell cycle biology and cancer associated abnormalities. This antibody is useful for
separating mantle cell lymphomas (cyclin D1 positive) from CLL/SLL and follicular lymphomas (cyclin D1 negative). Hairy cell leukemia and
plasma cell myeloma can weakly express Cyclin D1.
B-cell Lymphomas
Cyclin D1 Annexin
A1
MUM1 CD79a BCL2
BCL6
PD-1
CD10
CD23
CD5
Follicular
-
-
-
+
+
+
+
+
-
-
CLL/SLL
-
-
+
+
+
-
-
-
+
+
Mantle Cell
+
-
-/+
+
+
-
-
-
-
+
Marginal Zone
-
-
+
+
+
-
-
-
-
-
Lymphoplasmacytic
-
-
+
+
+
-
-
-
-
-
Diffuse Large Cell
-
-
+
+
+
+
-
-/+
-
-/+
Burkitt
-
-
-
+
-
+
-
+
-
-
Hairy Cell Leukemia
+(weak)/-
+
+
+
-
-
-
-
-
Plasma Cells
Cyclin D1 CD138
CD79a
EMA
MUM1
CD56
CD43
CD20
CD19
Plasma Cell Neoplasm
-/+
+
+
+
+
+
-
-/+
-
Reference
1. Mankin RC, Hunter SV. Arch Pathol Lab Med. 1999 Dec; 123(12):1182-8.
2. Yatabe Y, et al. Blood. 2000 Apr 1; 95(7):2253-61.
3. Kodet R, et al. Virchows Arch. 2003 Jun; 442(6):538-47. Epub 2003 May 01.
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