Advanced Solutions
for Advanced Pathology
Cytokeratin 5 + Cytokeratin 14 (EP1601Y
†
+ LL002)
Rabbit and Mouse Monoclonal Antibody
Cat. No. Description
Volume
45693 IMPATH CK5 + CK14 RTU M (EP1601Y & LL002)
50 Tests
44203 CK5 + CK14 RTU M (EP1601Y & LL002)
7 ml Ready To Use
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Esophagus, Squamous Cell
Carcinoma
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
1
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Cytokeratin 5 is an intermediate filament protein of 58 kD molecular wt. amongst the cytokeratin family. It is a type II (basic) cytokeratin.
Antibodies to this protein identify basal cells of squamous and glandular epithelia, myoepithelia, and mesothelium. Cytokeratin 14 is a 50 kD
polypeptide found in basal cells of squamous epithelia, some glandular epithelia, myoepithelium, and mesothelial cells.
Anti-Cytokeratin 5 has been useful in the differential diagnosis of metastatic carcinoma in the pleura versus epithelial mesothelioma. Almost all
squamous cell carcinomas, half of transitional carcinomas, and many undifferentiated large cell carcinomas immunostain with CK5. Anti-CK14
has been demonstrated to be useful in differentiating squamous cell carcinomas from other epithelial tumors. Anti-CK5, along with anti-CK14,
has found application in identifying the basaloid phenotype of breast carcinoma, a tumor with poor prognosis.
Reference
1. Clarke CL, et al. J Pathol. 2004 Oct; 204(2):147-52.
2. Comin CE, et al. Am J Surg Pathol. 2007 Aug; 31(8):1139-48.
3. Dabbs DJ, et al. Mod Pathol. 2006 Nov; 19(11); 1506-11. Epub 2006 Aug 25.
4. Livasy CA, et al. Hum Pathol. 2007 Feb; 38(2):197-204.
5. Reis-Filho JS, et al. Appl Immunohistochem Mol Morphol. 2003 Mar; 11(1):1-8.
6. Chu PG, et al. Histopathology. 2001 Jul; 39(1):9-16.
7. Chu PG, Weiss LM. Histopathology. 2001 Nov; 39(5):455-62.
99
