Antibodies for
Immunohistochemistry
Cytokeratin 7 (OV-TL 12/30)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45200 IMPATH Cytokeratin 7 RTU M (OV-TL 12/30)
50 Tests
44272 Cytokeratin 7 RTU M (OV-TL 12/30)
7 ml Ready To Use
44565 Cytokeratin 7 0,1 M (OV-TL 12/30)
100 µl liquid Concentrated
44566 Cytokeratin 7 1 M (OV-TL 12/30)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Lung adenocarcinoma, Salivary
gland
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
1
/K
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Anti-cytokeratin 7 reacts with proteins that are found in most ductal, glandular, and transitional epithelia of the urinary tract and bile duct epithelial
cells. Anti-cytokeratin 7 distinguishes between lung and breast epithelium that stain positive, and colon and prostate epithelial cells that are
negative. This antibody also reacts with many benign and malignant epithelial lesions, e.g. adenocarcinomas of the ovary, breast, and lung.
Transitional cell carcinomas are positive and prostate cancer is negative. This antibody does not recognize intermediate filament proteins.
Carcinomas
CK 7
CK
Cocktail
CK 20 GCDFP-15 ER/PR CK 5
p63
TTF-1
CEA CDX-2
Bladder Adenocarcinoma
+
+
+
-
-
-
-
-
+
+
Breast Carcinoma
+
+
-
+
+
-
-
-
-
-
Lung Adenocarcinoma
+
+
-
-
-
-
-
+
+
-
Endometrial
Adenocarcinoma
+
+
-
-
-
-
-
Ovarian Carcinoma
+
+
-
-
+
+
-
-
-
-
Cervical Carcinoma
+
+
-
-
-
-
-
-
+
-
Sweat Gland Carcinoma
+
+
-
-
-
+
+
+
-
Pancreatic Carcinoma
+
+
-
-
-
-
-
-
+
-
Gastric Carcinoma
+
+
-
-
-
-
-
-
+
+
Transitional Cell Carcinoma
+
+
+
-
-
+
+
-
-
-
Squamous Cell Carcinoma
-
+
-
-
+
+
+
-
-
-
Colon vs. Ovarian Carcinoma
CK 7
CK 20
CEA CDX-2
Villin CA19-9 Ep-CAM WT1 CA-125 CK 5
Ovarian Carcinoma, Serous
+
-
+
-
+
+
+
+
+
-
Ovarian Carcinoma,
Mucinous
+
-
-
+
+
+
+
-
-
Ovarian Carcinoma,
Endometrioid
+
-
-
-
+/-
+
+
+
-
Colorectal Carcinoma
-
+
+
+
+
+
+
-
-
-
Reference
1. Hatta N, Morita R, Yamada M, et al. Dermatol Surg. 2004 Oct; 30(10):1329-34.
2. Murray SK, Breau RH, Guha AK, Gupta R. Am J Surg Pathol. 2004 Sep; 28(9):1154-62.
3. Jerome MV, Mazieres J, Groussard O, et al. Histopathology. 2004 Aug; 45(2):125-34.
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