Antibodies for
Immunohistochemistry
Chromogranin A (LK2H10)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45195 IMPATH Chromogranin A RTU M (LK2H10)
50 Tests
44259 Chromogranin A RTU M (LK2H10)
7 ml Ready To Use
44539 Chromogranin A 0,1 M (LK2H10)
100 µl liquid Concentrated
44540 Chromogranin A 1 M (LK2H10)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Pancreas
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
1
/k
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Immunohistochemical methods have localized chromogranin in a wide variety of endocrine tissues such as the pituitary, pancreas, hypothalamus,
and parathyroid. Neuroendocrine cells exhibit a fine granular immunoreactivity to antichromogranin. It is generally accepted that the
co-expression of certain keratins and chromogranin mean neuroendocrine lineage. The presence of strong anti-chromogranin staining and
absence of anti-keratin staining should raise the possibility of paraganglioma. The co-expression of chromogranin and NSE is typical of
neuroendocrine neoplasms. Most pituitary adenomas and prolactinomas readily express chromogranin.
Retroperitoneal Neoplasms
Chromo-
granin A
NSE
Synapto-
physin Neurofilament
PGP 9.5
S-100
GFAP
CD99
Neuroblastoma
+
+
+
+
+
-
+/-
-
Ganglioneuroblastoma
+
+
+
+
+
+
+
-
Ganglioneuroma
+
+
+
+
+
+
+
-
Adrenal Tumors
Chromogranin A
Inhibin
Calretinin
MART-1
Synaptophysin
CD56
Pheochromocytoma
+
-
-
-
+
+
Adrenal Cortical Carcinoma
-
+
+
+
-/+
+
Adrenal Cortical Adenoma
-
+
+
+
-/+
+
Pancreas
Chromo-
granin A
Synapto-
physin CK 19 CA19-9 Gastrin E-cadherin CD10
CD56 β-Catenin S100P
Ductal Adenocarcinoma
-
-
+
+
-
+/-
+/-
-
+/-
+
Neuroendocrine Tumor
+
+
+/-
+/-
+/-
-
-
+
+
-
Solid Pseudopapillary
Tumor
-
+
-
-
-
+(nuclear)
+
+
+
-
Acinic Cell Carcinoma
-
-
+
-/+
-
+
+/-
-
+
-
Pancreatoblastoma
+
-
-
-
-
-
-
+
+
-
Benign Islet Cells
+
+
-
-
-
-
-
+
+
-
Benign Duct
-
-
-
-
-
-
-
-
-
-
Reference
1. Fischer-Colbrie R, et al. Neuroscience. 1985; 16:547.
2. Hearn SA. J Histochem Cytochem. 1987; 35:795-801.
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