Antibodies for
Immunohistochemistry
CD163 (MRQ-26)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45127 IMPATH CD163 RTU M (MRQ-26)
50 Tests
44223 CD163 RTU M (MRQ-26)
7 ml Ready To Use
44844 CD163 0,1 M (MRQ-26)
100 µl liquid Concentrated
44469 CD163 1 M (MRQ-26)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic, Membranous
Control
Inflamed Tissue
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
1
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
CD163 has been identified as an acute phase-regulated transmembrane protein whose function is to mediate the endocytosis of haptoglobin-
hemoglobin complexes. This receptor is expressed on the surface of monocytes with low expression and on tissue macrophages, histiocytes
with high expression.
Staining with anti-CD163 has been helpful to distinguish synovial macrophages from synovial intimal fibroblasts in the setting of rheumatoid
arthritis, where its specificity for macrophages was found to be superior to that of anti-CD68. Increased levels of CD163 were also detected
in patients with microbial infections and myelomonocytic leukemias by an enzyme-linked immunosorbent assay. Flow cytometry studies have
confirmed that CD163 expression is limited to leukemias with monocytic differentiation. Anti-CD163 can be used to identify and enumerate
myelomonocytic blasts and monocytic blasts in bone marrow biopsy for diagnosis of acute myeloid leukemia.
Mastocytosis
CD163
Tryptase
CD117
CD25
CD2
Systemic Mastocytosis
-
+
+
+
+
Reactive Mast Cells
+
+
+
-
-
Histiocytic Neoplasms
CD163
CD45
CD4
CD68
Lysozyme Factor XIIIa
CD20
CD3
Histiocytic Neoplasms
+
+
+
+
+
+
-
-
Skin: Dermatofibrosarcoma Protuberans (DF-SP) vs. Dermatofibroma Fibrous Histiocytoma (DF-FH)
CD163
CD34
NGFR
CD10 Factor XIIIa
p63
Desmin CK Cocktail
S-100
DF-SP
-
+
+
+/-
-
-
-
-
-
DF-FH
-
-
-
+
+
-
-
-
-
Reference
1. Backe E, et al. J Clin Pathol. 1991; 44:936-945.
2. Buechler C, et al. J Leukoc Biol. 2000; 67:97-103.
3. Hiraoka A, etal. Pathol Res Pract. 2005; 201 (5):379-84.
4. Hogger P, et al. J Immunol. 1998; 161:1883-1890.
5. Kristiansen M, et al. Nature. 2001; 409:198-201.
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