Advanced Solutions
for Advanced Pathology
Collagen Type IV (CIV22)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
44261 Collagen Type IV RTU M (CIV22)
7 ml Ready To Use
44543 Collagen Type IV 0,1 M (CIV22)
100 µl liquid Concentrated
44544 Collagen Type IV 1 M (CIV22)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Intercellular
Control
Lung, Muscle
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
1
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
Product Description
Collagen Type IV is the major component of the basal lamina so antibodies to this molecule confirm its presence and reveal the morphological
appearance of the structure. Normal tissue stains with this antibody in a fashion consistent with the sites of mesenchymal elements and
epithelial basal laminae. Anti-Collagen IV can also be useful in the classification of soft tissue tumors: schwannomas and leiomyomas. Their
well-differentiated, malignant counterparts usually immunoreact with this antibody. The vascular nature of neoplasms, hemangiopericytoma,
angiosarcoma and epithelioid hemangioendothelioma can be revealed by this antibody with greater reliability than non-specific stains (e.g. silver
reticulum).
Spindle Cell Melanoma vs. Epithelioid Peripheral Nerve Sheath Tumor
Collagen IV
S-100
HMB-45
Tyrosinase
NGFR
SOX-10
Spindle Cell Melanoma
-
+
+
+
+
+
PNST
+
+
+
+
+
+
Skin: Spindle Cell Tumors
Collagen
IV
FLI-1
S-100
CD99 Factor
XIIIa
HHV-8 CD10
CD34
D2-40 MS Actin
Squamous Cell Carcinoma
-
-
-
-
-
-
-
-
+
-
Spindle Cell Melanoma
-
+
+
-
-
-
-
-
+
-
Atypical Fibroxanthomas
-
-
-
+
+/-
-
+
-
-
+
DF-SP
-
-
-
-
-
-
+/-
+
-
-
DF-FH
-
-
-
-
+
-
+
-
-
-
Peripheral Nerve Sheath
-
-
+/-
+
-
-
-
-
+
+
Smooth Muscle
-
-
-
-/+
-
-
-
-
-
+
Angiosarcoma
+/-
+
-
-
-
-
-
+
+/-
-
Glomus Tumor
+
-
-
-
-
-
-
+/-
-
+
Solitary Fibrous Tumor
-
-/+
-
+/-
+/-
-
-
+
-
-
Hemangioma
+
+
-
-
-
-
-
+
-
-
Hemangioendothelioma
-
+
-
-
-
-
-
+
-
-
Kaposi's Sarcoma
+/-
+
-
-
-
+
-
+
+
-
Reference
1. Gould VE, et al. Pathol Annul. 1976; 11:353-386.
2. McArdle JP, et al. Int J Cancer. 1984; 34:633-638.
3. Sakr WA, et al. Hum Path. 1987; 18:1043-1050.
4. Barsky SH, et al. Am J Surg Pathol. 1983; 7:667-677.
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