Advanced Solutions
for Advanced Pathology
Claudin 1 (Polyclonal)
Rabbit Polyclonal Antibody
Cat. No. Description
Volume
45298 IMPATH Claudin 1 RTU R (Poly)
50 Tests
44260 Claudin1 RTU R (Poly)
7 ml Ready To Use
44541 Claudin1 0,1 R (Poly)
100 µl liquid Concentrated
44542 Claudin1 1 R (Poly)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Membranous
Control
Colon Carcinoma, Neurofibroma
Stability
Up to 36 mo. at 2-8°C
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
The claudins are a family of over twenty proteins which are components of tight junctions. Tight junctions are specialized regions of cell-to-cell
contact made up of a network of strands to act as a molecular ‘gasket’ for preventing the leakage of ions, water, etc. between cells. They are
abundant in luminal epithelial sheets where they maintain epithelial cell polarity. The claudins constitute a variable component, with specific
claudins being associated with specific tissues. The immunoreactivity for anti-claudin 1 is membranous and is found in nearly all carcinomas.
The staining is much stronger in the carcinoma cells than in normal tissues. Anti-claudin 1 in a panel of immunostains that includes antibodies
against EMA (positive), S-100 (negative), and GLUT1 can be utilized as a robust marker in the diagnosis of perineurioma and neurofibroma.
Some studies have shown anti-claudin 1 to be a specific marker for meningiomas. Therefore, anti-claudin 1 with anti-EMA, anti-S-100 protein,
anti-CD34, and anti-glial fibrillary acidic protein (GFAP) may be helpful in the differentiation of meningiomas from histologic mimics.
Perineurioma vs. Neurofibroma
Claudin 1
EMA
S-100
GLUT1
Perineurioma
+
+
-
+
Neurofibroma
+
+
+
-
Meningiomas from Histologic Mimics
Claudin 1
EMA
S-100
CD34
GFAP
Meningothelial Meningioma
+
+
-
-
-
Atypical Meningioma
+
+
-
+
-
Fibrous Meningioma
-
+
+
-
-
Solitary Fibrous Tumor
-
-
-
+
-
Meningeal
Hemangiopericytoma
-
-
-
+
-
Schwannoma
+/-
-
+
-
+
Reference
1. Folpe AL, et al. Am J Surg Pathol. 2002; 26:1620-6.
2. Hornick JL, Fletcher CD. Am J Surg Pathol. 2005; 29:845-58.
3. Y Soini. Histopathology. 2005; 47:551-560.
4. Smith MEF, et al. Histopathology. 2005; 47:575-581.
5. Liu Y, et al. Lung Cancer. 2007; 56(3):307-17.
6. Macarenco RS, et al. Arch Pathol Lab Med. 2007 Apr; 131(4)625-36.
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