Advanced Solutions
for Advanced Pathology
Cytokeratin (35betaH11)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45201 IMPATH Cytokeratin 8 RTU M (35betaH11)
50 Tests
44273 Cytokeratin 8 RTU M (35betaH11)
7 ml Ready To Use
44567 Cytokeratin 8 0,1 M (35betaH11)
100 µl liquid Concentrated
44568 Cytokeratin 8 1 M (35betaH11)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Prostate
Stability
Up to 36 mo. at 2-8°C
Isotype
IgM/k
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Cytokeratin 8 (CK8) belongs to a group of proteins known as intermediate-sized filaments that make up the cytoskeletal structure of virtually all
epithelial cells. Cytokeratin 8 is a basic (Type II) cytokeratin with a molecular weight of ~52 kDa. Type I and type II keratins heteropolymerize to
form in the cytoplasm of epithelial cells. This product typically dimerizes with cytokeratin 18 to form an intermediate filament in simple single-
layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular
differentiation. CK8 is present in simple epithelia and all adenocarcinomas.
CK8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as may be found in colon,
stomach, small intestine, trachea, and esophagus as well as in transitional epithelium. Anti-35betaH11 does not react with skeletal muscle
or nerve cells. Epithelioid sarcoma, chordoma, and adamantinoma express strong positivity corresponding to that of simple epithelia (with
antibodies against CK8, CK18 and CK19). Anti-35betaH11 has also been suggested as a marker for the differentiation of lobular (“ring-like,
perinuclear” ) from ductal (“peripheral-predominant”) carcinoma of the breast.
Epithelioid Cell Neoplasms
CK,
35βH11 INI-1
TFE-3
EMA CD34
FLI-1 Desmin
S-100 HMB-45 DOG1
Epithelioid Sarcoma
-
+
-
+
+
-
+
-
-
-
Epithelioid Angiosarcoma
+
+
-
-
+
+
-
-
-
-
MPNST
+
+/-
-
-
-/+
-
+
+
-
-
Leiomyosarcoma
+
-
-
-
-/+
+
-
-
-
GIST
-
-
-
-
+
-
-
-
-
+
Endothelial Tumors
-
+
-
-
+
+
-
-
-
-
PEComa
-
-
-
-
-
-
-
+
+
-
Clear Cell Sarcoma
+
-
-
-
-
-
-
+
-
-
Alveolar Soft Part Sarcoma
-
-
+
-
-
-
-
-
-
-
Melanoma
-
-
-
-
-
-
-
+
+
-
Plasmacytoma
+
-
-
+
-
-
-
-
-
-
Reference
1. Battifora H. Am J Surg Pathol. 1988; 12:24.
2. Gown AM, et al. Am J Clin Pathol. 1985; 84:413.
3. Knapp AC, et al. Cell. 1989; 59:67-79.
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