Antibodies for
Immunohistochemistry
Cytokeratin (34betaE12)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45138 IMPATH Cytokeratin RTU M (34betaE12)
50 Tests
44264 Cytokeratin RTU M (34betaE12)
7 ml Ready To Use
44549 Cytokeratin 0,1 M (34betaE12)
100 µl liquid Concentrated
44550 Cytokeratin 1 M (34betaE12)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Prostate
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
1
/k
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Anti-cytokeratin 34betaE12 is an antibody that recognizes cytokeratins 1, 5, 10, and 14 which are found in complex epithelia. Anti-cytokeratin
34betaE12 shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands; there is no reactivity with
cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are unreactive
with this antibody. Anti-cytokeratin 34betaE12 does label myoepithelial cells and has been shown to be useful in distinguishing prostate
adenocarcinoma from benign prostate. This antibody has also been useful in separating benign from malignant intraductal breast proliferations.
Prostate: Malignant vs. Benign
CK, 34βE12 PSA/PSAP Androgen
Receptor
P504s
p63
CK 5
CK 14
Prostate Carcinoma
-
+
+
+
-
-
-
Benign Prostate
+
+
+
-/+
+
+
+
Prostate Lesions
CK, 34βE12 PSA/PSAP P504s
p63
CK 7
Thrombo-
modulin Uroplakin III
PAX-2
Prostate Carcinoma
-
+
+
-
-
-
-
-
Urothelial Carcinoma
+
-
-
+
+
+
+
-
Nephrogenic Adenoma
+/-
-
+
-
+
-
-
+
Reference
1. Gown AM, et al. Am J Pathol. 1984; 114:309.
2. O’Malley FP, et al. Virch Arch A. 1990; 417:191.
3. Amin MB. Arch Pathol Lab Med. 1994 March; 118:260-264.
4. Wojno KJ, et al. Am J Surg Pathol. 1995; 19:251-60.
5. Moinfar F, et al. Am J Surg Pathol. 1999; 23:1048-58.
6. Yang XJ, et al. Am J Surg Pathol. 1999; 23:147-52.
94
