Advanced Solutions
for Advanced Pathology
Cytokeratin 8 & 18 (B22.1 & B23.1)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45202 IMPATH Cytokeratin 8 & 18 RTU M (B22.1 & B2) 50 Tests
44274 Cytokeratin 8&18 RTU M (B22.1&B23.1)
7 ml Ready To Use
44569 Cytokeratin 8&18 0,1 M (B22.1&B23.1)
100 µl liquid Concentrated
44570 Cytokeratin 8&18 1 M (B22.1&B23.1)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Pancreas, Prostate
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
1
/k & IgG
1
/k
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Cytokeratins 8 & 18 (CK 8 & 18) can be found in most simple epithelia, e.g. thyroid, female breast, gastrointestinal tract, and respiratory tract.
Adenocarcinomas and most non-keratinizing squamous carcinomas will stain with anti-CK 8 & 18, but keratinizing squamous carcinomas will
not. This antibody is used when attempting to demonstrate the presence of Paget cells; there is very little keratin 18 in the normal epidermis
so this will only stain Paget cells. The use of immunostaining facilitates the interpretation and has been shown to be more sensitive than mucin
histochemistry.
Skin: Spindle Cell Tumors
CK 8 & 18 FLI-1
HHV-8 CD34 Collagen
IV
D2-40 MS Actin CD10
S-100
CD99
Spindle Cell Squamous
Carcinoma
+
-
-
-
-
+
-
-
-
-
Spindle Cell Melanoma
-
+
-
-
-
+
-
-
+
-
Atypical Fibroxanthomas
-
-
-
-
-
-
+
+
-
+
DFSP
-
-
-
+
-
-
-
+/-
-
-
DF-FH
-
-
-
-
-
-
-
+
-
-
Peripheral Nerve Sheath
+
-
-
-
-
+
+
-
+/-
+
Smooth Muscle
-
-
-
-
-
-
+
-
-
-/+
Angiosarcoma
-
+
-
+
+/-
+/-
-
-
-
-
Glomus Tumor
-
-
-
+/-
+
-
+
-
-
-
Solitary Fibrous Tumor
-
-/+
-
+
-
-
-
-
-
+/-
Hemangioma
-
+
-
+
+
-
-
-
-
-
Hemangioendothelioma
+
+
-
+
-
-
-
-
-
-
Kaposi's Sarcoma
-
+
+
+
+/-
+
-
-
-
-
Reference
1. Moll R, et al. Histochem Cell Biol. 2008; 129:705-33.
2. Skinnider B. Am J Surg Pathol. 2005; 29:747-54.
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