Advanced Solutions
for Advanced Pathology
Nestin (10C2)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45653 IMPATH Nestin RTU M (10C2)
50 Tests
45640 Nestin RTU M (10C2)
7 ml Ready To Use
45624 Nestin 0,1 M (10C2)
100 µl liquid Concentrated
45625 Nestin 1 M (10C2)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Tonsil
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG1
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP 2-step Polymer (Universal) or AP
2-step Polymer (Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Nestin is a class VI intermediate filament (IF) protein. It has been reported that nestin expression is significantly increased in melanoma and
correlated with more advanced stages of the disease. An immunohistochemical analysis identified nestin-positive cells in 84% (35/42) of
primary melanoma and 83% (10/12) of metastatic melanoma. Nestin immunoreactivity was also observed in melanoma cells in all (10/10)
cases of HMB-45-negative amelanotic and melanotic, non-desmoplastic melanoma. Nestin expression has been reported in tumors of the
CNS, including astrocytoma, ependymoma, oligodendroglioma, glioblastoma, and primitive neuroectodermal tumors. Nestin has been detected
in human gliomas, including low and high grade, but its expression is observed more frequently in high grade than in low grade gliomas.
Nestin overexpression is seen in carcinomas, including prostatic adenocarcinoma, pancreatic ductal carcinoma, thyroid carcinoma, and in
mesenchymal tumors such as GIST and DFSP. Among the breast carcinoma subtypes, nestin is highly expressed in basal breast cancer
subtype (ERα-/PR-/Her2-) but not in the Her2 subtype (ERα-/PR-/Her2+) or luminal epithelial phenotype (ERα+/PR+). Only cytoplasmic staining
is considered positive, whereas any nuclear staining is considered as background artifact. In normal skin, nestin is observed in endothelial cells
and the bulge area of hair follicles.
Melanomas
Nestin
SOX-10
HMB-45
S-100
Desmoplastic Melanoma
+
+
-
+
Spindle Cell Melanoma
+
+
-/+
+
Conventional Melanoma
+
+
+
+
Reference
1. Brychtova S, et al. J CutanPathol. 2007; 34:370-375.
2. Maho KANOH, et al. Journal of Dermatology. 2010; 37:505-511.
3. Toshiyuki Ishiwata, et al. World J Gastroenterol. 2011; 17:409-418.
4. LI H, et al. Cancer Res. 2007; 67:501-510.
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