Advanced Solutions
for Advanced Pathology
Oct-4 (MRQ-10)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45245 IMPATH OCT-4 RTU M (MRQ-10)
50 Tests
44356 OCT-4 RTU M (MRQ-10)
7 ml Ready To Use
44732 OCT-4 0,1 M (MRQ-10)
100 µl liquid Concentrated
44733 OCT-4 1 M (MRQ-10)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Nuclear
Control
Seminoma
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Oct-4 is a transcription factor that maintains and regulates pluripotency in embryonic stem and germ cells. Anti-Oct-4 has shown a very high
sensitivity and specificity in seminoma/dysgerminoma, embryonal carcinoma, and the germ cell component of gonadoblastoma by nuclear
immunostaining. Clear cell carcinoma may enter the differential diagnosis of dysgerminoma as both may grow in nests or tubules, contain
clear cells, and have a prominent inflammatory infiltrate (lymphocytes in dysgerminoma and plasma cells in clear cell carcinoma). In one study
that looked at anti-Oct-4 staining in clear cell carcinomas, 4 of 14 tumors were found to be focally positive. In another study, 49 endometrioid
carcinomas were Oct-4 negative. Rarely dysgerminoma may have a morphologic appearance that overlaps with sex cord-stromal tumors,
especially poorly differentiated Sertoli cell tumors. In two studies however, all sex cord-stromal tumors of the testis and granulosa cell tumors of
the ovary were Oct-4 negative.
Germ Cell Tumors
Oct-4
AFP Vimentin EMA Inhibin
hPL
CD30 Glypican-3 CD117 PLAP
Seminoma
+
-
+
-
-
-
-
-
+
+
Embryonal Carcinoma
+
-
-
-
-
-
+
-
-
+
Choriocarcinoma
-
-
-/+
+
-
+
-
+
-
+
Yolk Sac Tumor
-
+
-
-
-
-
-
+
-
+
Granulosa Cell Tumor
-
-
+
-
+
-
-
-
-
-
Hypercalcaemic Small Cell
Carcinoma
-
-
-
+
-
-
-
-
-
-
Reference
1. Baker PM, Oliva E. Int J Gynecol Pathol. 2005 Jan; 24(1):39-55. Review.
2. Biermann K, et al. Histopathology. 2006 Sep; 49(3):290-7.
3. Cheng CJ, et al. J Biomed Sci. 2007 Nov; 14(6):797-807. Epub 2007 Aug 8.
4. Cools M, et al. J Clin Endocrinol Metab. 2006 Jun; 91(6):2404-13. Epub 2006 Apr 11.
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