Antibodies for
Immunohistochemistry
Neurofilament (2F11)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45242 IMPATH Neurofilament RTU M (2F11)
50 Tests
44352 Neurofilament RTU M (2F11)
7 ml Ready To Use
44724 Neurofilament 0,1 M (2F11)
100 µl liquid Concentrated
44725 Neurofilament 1 M (2F11)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Brain
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
1
/k
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Anti-neurofilament stains an antigen localized in a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas,
gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in
paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell
carcinomas of the lung also express neurofilament.
Retroperitoneal Neoplasms
Neuro-
filament
NSE
Synapto-
physin
Chromo-
granin A PGP 9.5
S-100
GFAP
CD99
Neuroblastoma
+
+
+
+
+
-
+/-
-
Ganglioneuroblastoma
+
+
+
+
+
+
+
-
Ganglioneuroma
+
+
+
+
+
+
+
-
Small Cell Carcinoma vs. Merkel Cell Carcinoma
Neuro-
filament
TTF-1
CEA
CK 20
Chromo-
granin A E-cadherin
CD117
Synapto-
physin
Merkel Cell Carcinoma
+
-
-
+
+
+(nuclear)
+
+
Small Cell Carcinoma
-
+
-
-
-
-
+/-
+
CNS Tumors
Neuro-
filament
GFAP Synapto-
physin S-100
CK
Cocktail
PR
EMA Vimentin NGFR INI-1
Astrocytoma
-
+
-
+
-
-
-
+
+
+
Glioblastoma
-
+
-
+
-
-
-
+
-
+
Oligodendriglioma
-
-
-
+
-
-
-
+
-
+
Ependymoma
-
+
-
+
-
-
-
-/+
+
+
Choroid Plexus Carcinoma
-
-/+
+
+
+
-
-
-
+
Central Neurocytoma
-
-
+
-
-
-
-
-
+
+
Neuroblastoma
+
+/-
+
+/-
-
-
-
+
+
+
Pineocytoma
-
-
+
-
-
-
-
-
+
Meningioma
-
-
-
-
-
+
+
+
-
+
Schwannoma
-
+
-
+
-
-
-
+
+
+
Rhabdoid Tumors
+/-
-
+/-
+
+
+
-
Metastatic Carcinoma
-
-
-
-
+
-/+
+
-/+
-
+
Reference
1. Wood JN, et al. Biosci. 1981; Rep, 1:263.
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