Antibodies for
Immunohistochemistry
SALL4 (6E3)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45690 IMPATH SALL4 RTU M (6E3)
50 Tests
45643 SALL4 RTU M (6E3)
7 ml Ready To Use
45629 SALL4 0,1 M (6E3)
100 µl liquid Concentrated
45630 SALL4 1 M (6E3)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Nuclear
Control
Seminoma, dysgerminoma
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
1
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
SALL4 is a zinc finger transcription factor. Anti-SALL4 demonstrates 100% sensitivity and stains more than 90% tumor cells in all intratubular
germ cell neoplasia, seminomas/dysgerminomas, embryonal carcinomas, and yolk sac tumor (YST) (both pediatric and postpubertal).
Anti-SALL4 also stains most cases of teratoma and the mononucleated trophoblastic cells in choriocarcinomas. In contrast, non-germ cell
tumors show no anti-SALL4 staining. Most non-testicular tumors from various organs and sites are negative for anti-SALL4, though an occasional
carcinoma or sarcoma may show weak anti-SALL4 staining in less than 25% of tumor cells. In normal testicular tissue, positive, weak anti-SALL4
staining is observed in spermatogonia. In addition, a few (<5%) primary spermatocytes showed dot-like weak anti-SALL4 staining. Secondary
spermatocytes, spermatids, spermatozoa, and Sertoli cells are negative for anti-SALL4. Leydig cells, rete testis, epididymis, spermatic cord
fibroblasts, blood vessels, and hematopoietic cells are negative for anti-SALL4.
Germ Cell Tumors
SALL4 SOX-2 Oct-4
PLAP CD117 CD30
AFP Beta-hCG Synapto-
physin Glypican
Seminoma/Dysgerminoma
+
-
+
+
+
-
-
-
-
-
Embryonal Carcinoma
+
+
+
+
-
+
-
-
-
-
Yolk Sac Tumor
+
-
-
-/+
-/+
-
+
-
-
+
Choriocarcinoma
-
-
-
+/-
-
-
-
+
-
-
Mature Teratoma
-
+/-
-
+/-
-
-
+/-
-
-
-
Immature Teratoma
+/-
+
-
-
+/-
-
-
+/-
-
-
Carcinoid
-
-
-
-
-
-
-
-
+
-
Reference
1. Cui W, et al. Mod. Pathol. 2006; 19:1585–1592.
2. Cao D, et al. Am J Surg Pathol. 2009; 33:894–904.
3. Cao D, et al. Am J Surg Pathol. 2009; 33:1065-1077.
214
