Advanced Solutions
for Advanced Pathology
S-100 (4C4.9)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45258 IMPATH S-100 RTU M (4C4.9)
50 Tests
44383 S-100 RTU M (4C4.9)
7 ml Ready To Use
44785 S-100 0,1 M (4C4.9)
100 µl liquid Concentrated
44786 S-100 1 M (4C4.9)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic, Nuclear
Control
Melanoma
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
2a
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
S-100 protein has been found in normal melanocytes, Langerhans cells, histiocytes, chondrocytes, lipocytes, skeletal and cardiac muscle,
Schwann cells, epithelial and myoepithelial cells of the breast, salivary and sweat glands, as well as in glial cells. Neoplasms derived from
these cells also express S-100 protein, albeit non-uniformly. A large number of well differentiated tumors of the salivary gland, adipose and
cartilaginous tissue, and Schwann cell-derived tumors express S-100 protein. Almost all malignant melanomas and cases of histiocytosis X are
positive for S-100 protein. Despite the fact that S-100 protein is an ubiquitous substance, its demonstration is of great value in the identification
of several neoplasms, particularly melanomas.
Melanotic Lesions
S-100
SOX-10 HMB-45 MART-1 Tyrosinase MiTF
CD63
WT1
NGFR
Junctional Nevus
+
+
+
+
+
+
-
+/-
Interdermal Nevus
+
+
-
+
+
+
-
+/-
Primary Melanoma
+
+
+
+
+
+
+
-
Spindle Cell Melanoma
+
+
+
+
+
+
+
+
+
Angiomyolipoma
+
+
+
+
+
+
+
Lymph Node
S-100
CD68
CD1a
Lysozyme
Langerhans Cell
Histiocytosis
+
+
+
+
Sinus Histiocytosis with
Massive Lymphadenopathy
+
+
-
+
Dermatopathic
Lymphadenitis
+
-
+
+
CNS Tumors
S-100
GFAP
Vimentin
NGFR
CK Cocktail
INI-1
Astrocytoma
+
+
+
+
-
+
Glioblastoma
+
+
+
-
-
+
Ependymoma
+
+
-/+
+
-
+
Schwannoma
+
+
+
+
-
+
Reference
1. Nakajima, et al. Ad J Surg Path. 1982; 6:715-727.
2. Kuhn, et al. Am J Clin Path. 1983; 79:341-347.
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