Advanced Solutions
for Advanced Pathology
Smoothelin (R4A)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45331 IMPATH Smoothelin RTU M (R4A)
50 Tests
44385 Smoothelin RTU M (R4A)
7 ml Ready To Use
44789 Smoothelin 0,1 M (R4A)
100 µl liquid Concentrated
44790 Smoothelin 1 M (R4A)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic, Cytoplasmic,
Nuclear
Control
Bladder, Leiomyoma
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
1
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Smoothelin is a constituent of the smooth muscle cell cytoskeleton protein exclusively found in differentiated smooth muscle cells (SMC). Cells
with SMC-like characteristics, such as myofibroblasts and myoepithelial cells, as well as skeletal and cardiac muscle do not contain smoothelin.
Distinguishing between bladder muscularis mucosae (MM) and muscularis propria (MP) muscle bundles is crucial for accurate staging of bladder
carcinoma. Strong smoothelin expression is nearly exclusively observed in muscularis propria. The staining pattern of MP (strongly positive) and
MM (negative or weakly positive) makes this technique an attractive diagnostic tool for the sometimes difficult task of staging bladder urothelial
carcinoma, such as in transurethral resection specimens of urinary bladder tumors. Differentiating between smooth muscle tumors and other
mesenchymal neoplasms of the GI tract can be challenging in small biopsies. Anti-smoothelin immunostaining can be helpful in differentiating
benign (+) from malignant smooth muscle tumors (-), and other mimics(-).
Bladder Tissue
Smoothelin
Actin, SM
Actin, MS
SMMHC
Calponin
Muscularis Mucosae
-
+
+
+
+
Muscularis Propria
+
+
+
+
+
Reference
1. Kramer J, et al. J Mol Med. 1999; 77:294-8.
2. van der Loop FT, et al. J Cell Biol. 1996; 134:401-411.
3. Maake C, et al. J Urol. 2006; 175:1152-1157.
4. Jimenez RE, et al. Adv Anat Pathol. 2000; 7:13-25.
5. Kuijpers, et al. Eur Urol. 2007; 52:1213-21.
6. Paner GP, et al. Am J Surg Pathol. 2009; 33:91-8.
7. Paner GP, et al. Am J Surg Pathol. 2010; 34:792-9.
8. Council L, et al. Mod Pathol. 2009; 22:639-650.
9. Coco DP, et al. Am J Surg Pathol. 2009 Dec; 33(12):1795-801.
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