Antibodies for
Immunohistochemistry
SOX-10 (Polyclonal)
Rabbit Polyclonal Antibody
Cat. No. Description
Volume
45332 IMPATH SOX-10 RTU R (Poly)
50 Tests
44387 SOX-10 RTU R (Poly)
7 ml Ready To Use
44793 SOX-10 0,1 R (Poly)
100 µl liquid Concentrated
44794 SOX-10 1 R (Poly)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Nuclear
Control
Melanoma, Schwannoma, Skin
Melanocytes
Stability
Up to 36 mo. at 2-8°C
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP 2-step Polymer (Universal) or
AP Polymer (Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Sry-related HMG-BOX gene 10, (SOX-10), a nuclear transcription factor that participates in neural crest development and in the specification and
differentiation of cells of melanocytic lineage, has been recently shown to be a sensitive marker of melanoma, including conventional, spindled,
and desmoplastic subtypes. SOX-10 nuclear expression was found in 76 of 78 melanomas (97%) and 38 of 77 malignant peripheral nerve sheath
tumors (49%), whereas S100 protein was expressed in 71 melanomas (91%) and 23 malignant peripheral nerve sheath tumors (30%). SOX-10
was expressed by metastatic melanomas and nodal capsular nevus in sentinel lymph nodes, but not by other lymph node components such as
dendritic cells which usually express S100 protein. It is known that the commonly used melanoma markers, anti-HMB-45 and anti-Melan-A, are
poorly expressed in desmoplastic melanomas while it has been reported that anti-SOX-10 was moderately to strongly expressed in all of the 28
desmoplastic melanomas tested. SOX-10 is less likely expressed by background fibrocytes and histiocytes in exicion scar than S100 and MiTF.
Therefore, anti-SOX-10 is considered a very reliable marker for recognizing residual demoplastic melanoma. SOX-10 is diffusely expressed in
schwannomas and neurofibromas. SOX-10 reaction is not identified in any other mesenchymal and epithelial tumors except for myoepitheliomas
and diffuse astrocytomas. SOX-10 expression is seen in sustentacular cells of pheochromocytomas and paragangliomas, and occasionally
carcinoid tumors from various organs, but not in the tumor cells. In normal tissues, SOX-10 is expressed in Schwann cells, melanocytes, and
myoepithelial cells of salivary, bronchial, eccrine, and mammary glands. SOX-10 expression is also observed in mast cells in a variety of tissues
and organs in both nuclear and cytoplasmic reaction.
Cutaneous Lesion
SOX-10
CK Cocktail
HMB-45
S-100
MART-1 (Melan A)
Conventional Melanoma
+
-/+
+
+
+
Desmoplastic Melanoma
+
-
-
+/-
-
Squamous Cell Carcinoma
-
+
-
-/+
-
Basal Cell Carcinoma
-
+
-
-
-
Merkel Cell Carcinoma
-
-/+
-
-/+
-
Lymph Node
SOX-10
CK Cocktail
HMB-45
S-100
MART-1 (Melan A)
Metastatic Melanoma
+
-
+
+
+
Nevus Cell
+
-
+
+
+
Interdigitating Dendritic
Cells
-
-
-
+
-
Reference
1. Kelsch RN. BioEssays. 2006; 28:788.
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