Advanced Solutions
for Advanced Pathology
T-bet (MRQ-46)
Rabbit Monoclonal Antibody
Cat. No. Description
Volume
44394 T-Bet RTU R (MRQ-46)
7 ml Ready To Use
44807 T-Bet 0,1 R (MRQ-46)
100 µl liquid Concentrated
44808 T-Bet 1 R (MRQ-46)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Nuclear
Control
Hairy Cell Leukemia, Tonsil
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
1
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
Product Description
T-bet, a T-box transcription factor, is expressed in CD4+ T-lymphocytes committed to T-helper (Th)1 T-cell development from naïve T-helper
precursor cells (Thp) and redirects Th2 T cells to Th1 development.
Anti-T-bet is a marker of mature T-cells and is expressed at very low levels in Th cells and is absent in precursor T-lymphoblastic leukemia/
lymphoma cells. Scattered small lymphocytes in the interfollicular T-cell zone of lymphoid tissue, including tonsil, lymph node, and spleen exhibit
nuclear staining for T-bet, with no T-bet staining being observed in germinal centers, mantle or marginal zones.
T-bet is expressed in a significant subset of B-cell lymphoproliferative disorders, particularly at an early stage of B-cell development (precursor
B-cell lymphoblastic leukemia/lymphoblastic lymphoma), and B-cell neoplasms derived from mature B cells, including CLL/SLL, marginal zone
lymphoma, and hairy cell leukemia. In contrast, B-cell neoplasms derived from pregerminal center or germinal center B-cells, including mantle
cell lymphoma, follicular lymphoma, diffuse large B-cell lymphoma, and Burkitt lymphoma are negative for T-bet. Anti-T-bet is a useful marker
for the diagnosis and subtyping of B-cell and T-cell lymphoproliferative disorders. T-bet is not expressed in precursor T-cell lymphoblastic
lymphoma/leukemia.
B-cell Lymphomas
T-bet
CD79a
BCL2
BCL6
CD10
CD23 Cyclin D1
IgD
MUM1
Follicular
-
+
+
+
+
-
-
+
-/+
CLL/SLL
+/-
+
+
-
-
+
-
+
+
Mantle Cell
-
+
+
-
-
-
+
+
-
Marginal Zone BCL
+
+
+
-
-
-
-
-/+
+
Lymphoplasmacytic
+
+
+
-
-
-
-
-
+
Diffuse Large Cell
Lymphoma
-
+
+
+
-
-
-
-
+
Burkitt Lymphoma
-
+
-
+
+
-
-
-
-
Hairy Cell Leukemia
+
+
+
-
-
-
+(weak)/-
-
Reference
1. Szabo SJ, et al. Cell. 2000; 100(6):665-69.
2. Zhang WX, et al. Genomics. 2001; 70(1):41-8.
3. Johrens K, et al. Am J Surg Pathol. 2007 Aug; 31(8):1181-1185.
4. Atayar C, et al. Am J Pathol. 2005; 166:127-134.
5. Dorfman DM, et al. Am J Clin Pathol. 2004; 122:292-297.
6. Harashima A, et al. Leuk Res. 2005 Jul; 29(7):841-8.
7. Marafioti T, et al. Am J Pathol. 2003; 162:861-871.
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