Antibodies for
Immunohistochemistry
Synaptophysin (MRQ-40)
Rabbit Monoclonal Antibody
Cat. No. Description
Volume
45260 IMPATH Synaptophysin RTU R (MRQ40)
50 Tests
44392 Synaptophysin RTU R (MRQ-40)
7 ml Ready To Use
44803 Synaptophysin 0,1 R (MRQ-40)
100 µl liquid Concentrated
44804 Synaptophysin 1 R (MRQ-40)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Pancreatic islet cells
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
1
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Anti-synaptophysin reacts with neuroendocrine cells of human adrenal medulla, carotid body, skin, pituitary, thyroid, lung, pancreas, and
gastrointestinal mucosa. Positive staining is seen in neurons of the brain, spinal cord, retina, Paneth cells in the gastrointestinal tract, and gastric
parietal cells. This antibody identifies normal neuroendocrine cells and neuroendocrine neoplasms. Diffuse, finely granular cytoplasmic staining
is observed, which probably correlates with the distribution of the antigen within neurosecretory vesicles. The expression of synaptophysin is
independent of the presence of NSE or other neuroendocrine markers. Anti-synaptophysin is an independent, broad-range marker of neural and
neuroendocrine differentiation.
Adrenal Tumors
Synaptophysin
Inhibin
Calretinin
MART-1
Chromogranin A
CD56
Pheochromocytoma
+
-
-
-
+
+
Adrenocortical Carcinoma
-/+
+
+
+
-
+
Adrenocortical Adenoma
-/+
+
+
+
-
+
CNS Tumors
Synapto-
physin
GFAP
Neuro-
filament
S-100 CK Cocktail
EMA Vimentin
NGFR
INI-1
Central Neurocytoma
+
-
-
-
-
-
-
+
+
Neuroblastoma
+
+/-
+
+/-
-
-
+
+
+
Pineocytoma
+
-
-
-
-
-
-
+
Metastatic Carcinoma
-
-
-
-
+
+
-/+
-
+
Pancreas
Synapto-
physin
Chromo-
granin A Insulin Glucagon Gastrin Somato-
statin
CD10
CD56 β-Catenin PGP 9.5
Neuroendocrine Tumor
+
+
+/-
+/-
+/-
+/-
-
+
+
+
Solid Pseudopapillary
Tumor
+
-
-
-
-
+
+
+
-
Normal Pancreas
+
+
+
+
-
+
-
-
+
-
Reference
1. Navone F, et al. J Cell Biol. 1986; 103:2511-2527.
2. Wiedenmann B, et al. Cell. 1985; 41:1017-1028.
3. Kayser K, et al. Path Res Pract. 1988; 183:412-417.
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