Antibodies for
Immunohistochemistry
BOB.1 (SP92)
Rabbit Monoclonal Antibody
Cat. No. Description
Volume
45124 IMPATH BOB-1 RTU R (SP92)
50 Tests
44202 BOB-1 RTU R (SP92)
7 ml Ready To Use
44431 BOB-1 0,1 R (SP92)
100 µl liquid Concentrated
44432 BOB-1 1 R (SP92)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic, Nuclear
Control
Tonsil
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
1
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution Buffer
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP 2-step Polymer (Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
The BOB.1/OBF.1 coactivator has shown to be a critical determinant of octamer-dependent gene transcription in B-lymphocytes. Expression
of BOB.1/OBF.1 is restricted largely to B-lymphocytes. Analyses of BOB.1/OBF.1 expression in a variety of established B-cell lines representing
different stages of B-cell development has suggested a constitutive, B-cell specific expression pattern. Interestingly, expression of BOB.1/OBF.1
can be induced in T-lymphocytes by costimulation with phorbol ester (PMA) and ionomycin. BOB.1 is expressed in germinal center B-cells,
mantle B-cells, and plasma cells. Various lymphomas are also positive for this marker including the following: B-chronic lymphocytic leukemia,
mantle cell lymphoma, follicular lymphoma, marginal zone lymphoma, plasmacytoma, Burkitt lymphoma, diffuse large cell lymphoma, diffuse
large B-cell lymphoma, T-cell rich B-cell lymphoma, and nodular lymphocyte predominant Hodgkin lymphoma.
B-cell Lymphomas
BOB.1 MUM1
PU.1
CD79a
p27
BCL6
IgD CD10
CD23 Cyclin D1
Follicular
+
-
+
+
+
+
+
+
-
-
CLL/SLL
+
+
+
+
+
-
+
-
+
-
Mantle Cell
+
-
+
+
+
-
+
-
-
+
Marginal Zone BCL
+
+
+
+
+
-
-/+
-
-
-
Lymphoplasmacytic
+
+
+
+
-
-
-
-
-
Diffuse Large Cell
Lymphoma
+
+
+
+
-
+
-
-
-
-
Hodgkin vs. Non-Hodgkin Lymphomas
BOB.1 CD45 MUM1
EMA CD15
CD30 Fascin BCL6
Oct-2
PU.1
Hodgkin Lymphoma,
Classic
-
-
+
-
+
+
+
-
-
-
Hodgkin Lymphoma,
Nodular Lymphocyte
Predominant
+
+
-/+
+
-
-
-
+
+
+
T-cell Rich LBCL
+
+
+
-
-
-
-
+
+
-
Acute Myeloid Leukemia
BOB.1
MPO Factor VIII
CD61
Oct-2
CD43
CD138
CD68
Promyelocytic, M3
+
+
-
-
+
+
+
Megakaryoblastic, M7
+/-
-
+
+
-
-
-
Reference
1. Dabbs DJ. Diagnositc Immunohistochemistry, Third Edition. Saunders. 2006.
2. Steimle-Grauer SA, et al. Virchows Arch. 2003; 442:284-293.
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