Antibodies for
Immunohistochemistry
Cytokeratin 19 (A53-B/A2.26)
Mouse Monoclonal Antibody
Cat. No. Description
Volume
45199 IMPATH Cytokeratin 19 RTU M (A53-B/A2.26) 50 Tests
44268 Cytokeratin 19 RTU M (A53-B/A2.26)
7 ml Ready To Use
44557 Cytokeratin 19 0,1 M (A53-B/A2.26)
100 µl liquid Concentrated
44558 Cytokeratin 19 1 M (A53-B/A2.26)
1 ml liquid Concentrated
Product Specifications
Designation
IVD
Reactivity
Paraffin
Visualization
Cytoplasmic
Control
Bladder, Colon, Colon carcinoma,
Thyroid carcinoma
Stability
Up to 36 mo. at 2-8°C
Isotype
IgG
2a
Manual Protocol*
• Pretreatment: Heat Induced Epitope
Retrieval (HIER)
• Primary Antibody Incubation Time:
10-30min @ 25-37°C
• 2-step polymer detection
*Please refer to product insert for complete protocol.
ImPath Protocol*
• Dewax: Dewax Solution 2 (DS2)
• Pretreatment: Retrieval Solution pH 9.0
(TR1) 32min @ 98-103°C
• Primary Antibody Incubation Time:
10-90min @ 25-37°C
• HRP Polymer (Universal) or AP Polymer
(Universal) for 12 min
*Please refer to product insert for complete protocol.
Product Description
Anti-cytokeratin 19 reacts with a wide variety of epithelia and epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas,
biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although follicular
carcinoma is also labeled by this antibody approximately 50%-60% of the time.
Thyroid: Malignant vs. Benign
CK 19
Thyroglobulin
Calcitonin
Galectin-3
TTF-1
HBME-1
Papillary Carcinoma
+
+
-
+
+
+
Follicular Carcinoma
-/+
+
-
+
+
+/-
Medullary Carcinoma
+/-
-
+
-
+
+
Benign Thyroid
-
+
-
-
+
-
Cutaneous Neoplasm
CK 19
CD10
Androgen
Receptor
CK 20
CD34
Ber-EP4
BCL2
Basal Cell Carcinoma
+
+
+
-
-
+
+
Trichoepithelioma
+
-
-
+
+
+
+
Merkel Cell Carcinoma
+
-
-
+
-
+
+
Microcystic Adnexal
Carcinoma
+/-
-
-
-
-/+
+
Sebaceous Carcinoma
-
+/-
+
-
-
+
+/-
Sebaceous Adenoma
-
-
+
-
-
+
+
Pancreas
CK 19 Synapto-
physin
Chromo-
granin A E-cadherin CD10 Gastrin CA19-9 CD56 β-Catenin S100P
Neuroendocrine Tumor
+/-
+
+
-
-
+/-
+/-
+
+
-
Solid Pseudopapillary
Tumor
-
+
-
+(nuclear)
+
-
-
+
+
-
Ductal Carcinoma
+
-
-
+/-
+/-
-
+
-
+/-
+
Acinic Cell Carcinoma
+
-
-
+
+/-
-
-/+
-
+
-
Pancreatoblastoma
-
-
+
-
-
-
-
+
+
-
Normal Pancreas
-
+
+
-
-
-
-
-
+
-
Reference
1. Zubair W, et al. Hum Pathol. 30:1166-1171.
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